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aa08e12.s1 Soares_NhHMPu_S1 Homo sapiens cDNA clone IMAGE:812686 3', mRNA sequence

GenBank: AA463897.1

FASTA Graphics 

LOCUS       AA463897                 175 bp    mRNA    linear   EST 10-JUN-1997
DEFINITION  aa08e12.s1 Soares_NhHMPu_S1 Homo sapiens cDNA clone IMAGE:812686
            3', mRNA sequence.
ACCESSION   AA463897
VERSION     AA463897.1
DBLINK      BioSample: SAMN00155192
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 175)
  AUTHORS   Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S.,
            Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin,J.,
            Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B.,
            White,Y., Wylie,T., Waterston,R. and Wilson,R.
  TITLE     WashU-Merck EST Project 1997
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: -41m13 fwd. ET from Amersham
            High quality sequence stop: 163.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:6043596"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:812686"
                     /tissue_type="Pooled human melanocyte, fetal heart, and
                     pregnant uterus"
                     /clone_lib="SAMN00155192 Soares_NhHMPu_S1"
                     /lab_host="DH10B"
                     /note="Organ: mixed (see below); Vector: pT7T3D-PacI;
                     Site_1: Not I; Site_2: Eco RI; Equal amounts of plasmid
                     DNA from three normalized libraries (melanocyte 2NbHM,
                     pregnant uterus NbHPU, and fetal heart NbHH19W) were
                     mixed, and ss circles were made in vitro. Following HAP
                     purification, this DNA was used as tracer in a subtractive
                     hybridization reaction. The driver was PCR-amplified cDNAs
                     from pools of 5,000 clones made from the same 3 libraries.
                     The pools consisted of I.M.A.G.E. clones 260232-265223,
                     340488-345479, and 484488-489479."
ORIGIN      
        1 aaggataaca tagatttatg tttatttgca ttttatgttt ccttatggtt catatgagtt
       61 tattggaaaa ttagcaacat acacatcatt tgggggaatg gaaagtgagg cagagtaagc
      121 cagaacattc actgtaacat tataagcaag tagaataaat tgataatacc ttcac
//
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