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GenBank: AA504338.1
FASTA Graphics
LOCUS AA504338 528 bp mRNA linear EST 08-JAN-2011 DEFINITION aa61f06.s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:825443 3', mRNA sequence. ACCESSION AA504338 VERSION AA504338.1 DBLINK BioSample: SAMN00155198 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 528) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Louis M. Staudt, M.D., Ph.D., David Allman, Ph.D., Gerald Marti, M.D. cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima Bonaldo, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 486. FEATURES Location/Qualifiers source 1..528 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:825443" /tissue_type="germinal center B cell" /clone_lib="SAMN00155198 NCI_CGAP_GCB1" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was prepared from human tonsillar cells enriched for germinal center B cells by flow sorting (CD20+, IgD-), provided by Dr. Louis M. Staudt (NCI), Dr. David Allman (NCI) and Dr. Gerald Marti (CBER). cDNA synthesis was primed with a Not I - oligo(dT) primer [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCTCATTTTTTTTTTTTTTTTTT- 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo." ORIGIN 1 tttttttttg tcttccaggg taacagaaaa tggattaaga ataaaaattc caagtttcag 61 tgtcacatat aagtcatata tatcaatgta aatgttagta taaggatgaa acactgaggg 121 caactattaa ggcatttcag atttcagtca tgaaatttga catatcatat tcttaatcct 181 aattgtcaag ctaagagtgg aagggataaa acaaattcta aaaatcagtc ttatttaact 241 ctttctacac tacagataac attatatcct ctctaaattt cagtgtataa tcttttaagc 301 aaaattagca tacatattgt tttcatacta ttaggttatt ttcaaaggta caaaaagcaa 361 ggtaaatcaa atactaatta catcatattg gcctcaaact ttcttaaaac ttcagttctt 421 gttacacaga gaaataatta aggttcttat aactgggctt cgtattttgc tatttttgtt 481 ccctacacat aactggagat gataggtgga ctaaaccaag tgaagaaa //
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