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aa61f06.s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:825443 3', mRNA sequence

GenBank: AA504338.1

FASTA Graphics 

LOCUS       AA504338                 528 bp    mRNA    linear   EST 08-JAN-2011
DEFINITION  aa61f06.s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:825443 3',
            mRNA sequence.
ACCESSION   AA504338
VERSION     AA504338.1
DBLINK      BioSample: SAMN00155198
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 528)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: Louis M. Staudt, M.D., Ph.D., David Allman,
            Ph.D., Gerald Marti, M.D.
             cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima
            Bonaldo, Ph.D.
             cDNA Library Arrayed by: Greg Lennon, Ph.D.
             DNA Sequencing by: Washington University Genome Sequencing Center
             Clone distribution: NCI-CGAP clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            www-bio.llnl.gov/bbrp/image/image.html
            
            Seq primer: -40m13 fwd. ET from Amersham
            High quality sequence stop: 486.
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:825443"
                     /tissue_type="germinal center B cell"
                     /clone_lib="SAMN00155198 NCI_CGAP_GCB1"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was prepared from human tonsillar cells
                     enriched for germinal center B cells by flow sorting
                     (CD20+, IgD-), provided by Dr. Louis M. Staudt (NCI), Dr.
                     David Allman (NCI) and Dr. Gerald Marti (CBER). cDNA
                     synthesis was primed with a Not I - oligo(dT) primer
                     [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCTCATTTTTTTTTTTTTTTTTT-
                     3']. Double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization, and was
                     constructed by Bento Soares and M. Fatima Bonaldo."
ORIGIN      
        1 tttttttttg tcttccaggg taacagaaaa tggattaaga ataaaaattc caagtttcag
       61 tgtcacatat aagtcatata tatcaatgta aatgttagta taaggatgaa acactgaggg
      121 caactattaa ggcatttcag atttcagtca tgaaatttga catatcatat tcttaatcct
      181 aattgtcaag ctaagagtgg aagggataaa acaaattcta aaaatcagtc ttatttaact
      241 ctttctacac tacagataac attatatcct ctctaaattt cagtgtataa tcttttaagc
      301 aaaattagca tacatattgt tttcatacta ttaggttatt ttcaaaggta caaaaagcaa
      361 ggtaaatcaa atactaatta catcatattg gcctcaaact ttcttaaaac ttcagttctt
      421 gttacacaga gaaataatta aggttcttat aactgggctt cgtattttgc tatttttgtt
      481 ccctacacat aactggagat gataggtgga ctaaaccaag tgaagaaa
//
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