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zi34a05.s1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE:432656 3', mRNA sequence

GenBank: AA700547.1

FASTA Graphics 

LOCUS       AA700547                 441 bp    mRNA    linear   EST 19-DEC-1997
DEFINITION  zi34a05.s1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA
            clone IMAGE:432656 3', mRNA sequence.
ACCESSION   AA700547
VERSION     AA700547.1
DBLINK      BioSample: SAMN00155062
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S.,
            Krizman,D., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M.,
            Martin,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F.,
            Theising,B., White,Y., Wylie,T., Waterston,R. and Wilson,R.
  TITLE     WashU-NCI human EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: -40m13 fwd. ET from Amersham
            High quality sequence stop: 391.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:1332425"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:432656"
                     /sex="male"
                     /clone_lib="SAMN00155062
                     Soares_fetal_liver_spleen_1NFLS_S1"
                     /dev_stage="20 week-post conception fetus"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
                     with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
                     This is a subtracted version of the original Soares fetal
                     liver spleen 1NFLS library. 1st strand cDNA was primed
                     with a Pac I - oligo(dT) primer [5'
                     AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Pac I and cloned into the Pac I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization. Library
                     constructed by Bento Soares and M.Fatima Bonaldo."
ORIGIN      
        1 tttttttttt tttatttatc aacagtatat ttgacaaaaa ccacccccaa tcgatggcag
       61 aaagcagtat tttctagctg gcactgaaac ttaacctatt tttttgaggg agaaaaagac
      121 taaaatgtaa gcactaactt cctccttctg catcttccac agaagaccca cttgtgggca
      181 ttctctttct gtccgttatc agtgggttta catccgaagg ccggcggaga gacacttgaa
      241 ccacaaacac cagacatgca ggtgtctcaa tggcatgcag attatttaaa gtgcatcact
      301 tgtgagaagc ttctcaaatt tctgactgtc ataaccaggt gtcacctaaa atctgtcctg
      361 taggccaaga tgctacacgc agcgcccagg cagtcagagg taagtgagcg cctcccgcct
      421 cagaaagctt ttacagggcc a
//
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