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GenBank: AA700547.1
FASTA Graphics
LOCUS AA700547 441 bp mRNA linear EST 19-DEC-1997 DEFINITION zi34a05.s1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE:432656 3', mRNA sequence. ACCESSION AA700547 VERSION AA700547.1 DBLINK BioSample: SAMN00155062 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 441) AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S., Krizman,D., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B., White,Y., Wylie,T., Waterston,R. and Wilson,R. TITLE WashU-NCI human EST Project JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 391. FEATURES Location/Qualifiers source 1..441 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="GDB:1332425" /db_xref="taxon:9606" /clone="IMAGE:432656" /sex="male" /clone_lib="SAMN00155062 Soares_fetal_liver_spleen_1NFLS_S1" /dev_stage="20 week-post conception fetus" /lab_host="DH10B (ampicillin resistant)" /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Pac I; Site_2: Eco RI; This is a subtracted version of the original Soares fetal liver spleen 1NFLS library. 1st strand cDNA was primed with a Pac I - oligo(dT) primer [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Pac I and cloned into the Pac I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by Bento Soares and M.Fatima Bonaldo." ORIGIN 1 tttttttttt tttatttatc aacagtatat ttgacaaaaa ccacccccaa tcgatggcag 61 aaagcagtat tttctagctg gcactgaaac ttaacctatt tttttgaggg agaaaaagac 121 taaaatgtaa gcactaactt cctccttctg catcttccac agaagaccca cttgtgggca 181 ttctctttct gtccgttatc agtgggttta catccgaagg ccggcggaga gacacttgaa 241 ccacaaacac cagacatgca ggtgtctcaa tggcatgcag attatttaaa gtgcatcact 301 tgtgagaagc ttctcaaatt tctgactgtc ataaccaggt gtcacctaaa atctgtcctg 361 taggccaaga tgctacacgc agcgcccagg cagtcagagg taagtgagcg cctcccgcct 421 cagaaagctt ttacagggcc a //
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