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oa52g10.r1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:1308642 5', mRNA sequence

GenBank: AA743855.1

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LOCUS       AA743855                 456 bp    mRNA    linear   EST 06-JAN-2011
DEFINITION  oa52g10.r1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:1308642 5',
            mRNA sequence.
ACCESSION   AA743855
VERSION     AA743855.1
DBLINK      BioSample: SAMN00155198
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 456)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: Louis M. Staudt, M.D., Ph.D., David Allman,
            Ph.D., Gerald Marti, M.D.
             cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima
            Bonaldo, Ph.D.
             cDNA Library Arrayed by: Greg Lennon, Ph.D.
             DNA Sequencing by: Washington University Genome Sequencing Center
             Clone distribution: NCI-CGAP clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            www-bio.llnl.gov/bbrp/image/image.html
            
            Insert Length: 727   Std Error: 0.00
            Seq primer: -28m13 rev1 ET from Amersham
            High quality sequence stop: 446.
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:1308642"
                     /tissue_type="germinal center B cell"
                     /clone_lib="SAMN00155198 NCI_CGAP_GCB1"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was prepared from human tonsillar cells
                     enriched for germinal center B cells by flow sorting
                     (CD20+, IgD-), provided by Dr. Louis M. Staudt (NCI), Dr.
                     David Allman (NCI) and Dr. Gerald Marti (CBER). cDNA
                     synthesis was primed with a Not I - oligo(dT) primer
                     [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCTCATTTTTTTTTTTTTTTTTT-
                     3']. Double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization, and was
                     constructed by Bento Soares and M. Fatima Bonaldo."
ORIGIN      
        1 ggcaaaagta aatacacaga caaattgaga atattttaac actgtagttc tggtgtgtga
       61 acccctcata tctttagtgt gagcactaaa atacaaatct gtcaagaatg atgactgaat
      121 agctttttag gagatatata aaaagatata aagtgagaca acgaaaagaa aaggagtgat
      181 gagttatagt gtagagattt ttagtttatt ctttgcttat ttcttttcat tgtgatcaaa
      241 gtcaagttgt catcagttta aaataacttg ctgtaactgt aagatacttt ttatgtctca
      301 tggcaaccat aatgcaaaaa tttatagtag agatactaaa aacaaaaagc aaaggattaa
      361 atcatactac cagagaaaat cacttatcca caaaaaaggg agtaagaaaa ttagagaaga
      421 cttagaagac aaataacaaa atggaagtag taagtc
//
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