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GenBank: AA743855.1
FASTA Graphics
LOCUS AA743855 456 bp mRNA linear EST 06-JAN-2011 DEFINITION oa52g10.r1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:1308642 5', mRNA sequence. ACCESSION AA743855 VERSION AA743855.1 DBLINK BioSample: SAMN00155198 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 456) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Louis M. Staudt, M.D., Ph.D., David Allman, Ph.D., Gerald Marti, M.D. cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima Bonaldo, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 727 Std Error: 0.00 Seq primer: -28m13 rev1 ET from Amersham High quality sequence stop: 446. FEATURES Location/Qualifiers source 1..456 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:1308642" /tissue_type="germinal center B cell" /clone_lib="SAMN00155198 NCI_CGAP_GCB1" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was prepared from human tonsillar cells enriched for germinal center B cells by flow sorting (CD20+, IgD-), provided by Dr. Louis M. Staudt (NCI), Dr. David Allman (NCI) and Dr. Gerald Marti (CBER). cDNA synthesis was primed with a Not I - oligo(dT) primer [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCTCATTTTTTTTTTTTTTTTTT- 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo." ORIGIN 1 ggcaaaagta aatacacaga caaattgaga atattttaac actgtagttc tggtgtgtga 61 acccctcata tctttagtgt gagcactaaa atacaaatct gtcaagaatg atgactgaat 121 agctttttag gagatatata aaaagatata aagtgagaca acgaaaagaa aaggagtgat 181 gagttatagt gtagagattt ttagtttatt ctttgcttat ttcttttcat tgtgatcaaa 241 gtcaagttgt catcagttta aaataacttg ctgtaactgt aagatacttt ttatgtctca 301 tggcaaccat aatgcaaaaa tttatagtag agatactaaa aacaaaaagc aaaggattaa 361 atcatactac cagagaaaat cacttatcca caaaaaaggg agtaagaaaa ttagagaaga 421 cttagaagac aaataacaaa atggaagtag taagtc //
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