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GenBank: AA748488.1
FASTA Graphics
LOCUS AA748488 443 bp mRNA linear EST 07-JAN-2011 DEFINITION ny01g06.s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE:1270522 3', mRNA sequence. ACCESSION AA748488 VERSION AA748488.1 DBLINK BioSample: SAMN00155198 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 443) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Louis M. Staudt, M.D., Ph.D., David Allman, Ph.D., Gerald Marti, M.D. cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima Bonaldo, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 699 Std Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 433. FEATURES Location/Qualifiers source 1..443 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:1270522" /tissue_type="germinal center B cell" /clone_lib="SAMN00155198 NCI_CGAP_GCB1" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was prepared from human tonsillar cells enriched for germinal center B cells by flow sorting (CD20+, IgD-), provided by Dr. Louis M. Staudt (NCI), Dr. David Allman (NCI) and Dr. Gerald Marti (CBER). cDNA synthesis was primed with a Not I - oligo(dT) primer [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCTCATTTTTTTTTTTTTTTTTT- 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo." ORIGIN 1 tttttttttc ctggagttaa tcattgatcc atttaaaaac ttcttgtttt ttttaatgac 61 ctatctgcac acttggatag gactggtaaa ttacaagcac tcccagtcac atcaggttat 121 ctatccattc ctttttttcc cacccccctt ggagacccac tccttggagt ccttcctgct 181 ccagtctgac tgcttttgct ctccaggcct gctgcctagt tgtcatctgg agcttcatca 241 tcatccttgg gattcccttt gcctgtcttc tctggtgggt ctcctgtttc ctacaatctt 301 agtcgcctcc tgagaaagga taaacctttg ggttcttatc tgaaaatgcc ttagtttatc 361 ctaattgatt aatagtttag ctgggtgtgg cattctaagt tggaaatcat tttcttggat 421 aattttgaag gaactgtcat ctt //
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