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UI-R-C0-in-a-03-0-UI.s1 UI-R-C0 Rattus norvegicus cDNA clone UI-R-C0-in-a-03-0-UI 3', mRNA sequence

GenBank: AA999071.1

FASTA Graphics 

LOCUS       AA999071                 375 bp    mRNA    linear   EST 26-JAN-2011
DEFINITION  UI-R-C0-in-a-03-0-UI.s1 UI-R-C0 Rattus norvegicus cDNA clone
            UI-R-C0-in-a-03-0-UI 3', mRNA sequence.
ACCESSION   AA999071
VERSION     AA999071.1
DBLINK      BioSample: SAMN00155877
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     On Jun 5, 1998 this sequence version replaced gi:3189662.
            Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            Oligo-dT track not found, Not I site shown in beginning of sequence
            is likely internal to the message. cDNA Library Preparation: M.
            Fatima Bonaldo, Ph.D. Clone distribution: clones will be available
            through Research Genetics
            Seq primer: M13 Forward.
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-C0-in-a-03-0-UI"
                     /clone_lib="SAMN00155877 UI-R-C0"
                     /dev_stage="adult"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-C0 library is a subtracted library derived from
                     the UI-R-A1 and UI-R-E1 libraries. The UI-R-A1 library
                     consisted of a mixture of individually tagged normalized
                     libraries constructed from rat placenta, adult lung,
                     brain, liver, kidney, heart, spleen, ovary, and muscle.
                     The UI-R-E1 library consisted of a mixture of individually
                     tagged normalized libraries constructed from 8, 12 and
                     18-day embryo. The tag is a string of 3-5 nucleotides
                     present between the Not I site and the oligo-dT track
                     which allows identification of the library of origin of a
                     clone within the mixture. The subtracted library (UI-R-C0)
                     was constructed as follows: PCR amplified cDNA inserts
                     from a pool of UI-R-A1 and UI-R-E1 clones from which 3'
                     ESTs had been derived was used as a driver in a
                     hybridization with the pooled UI-R-A1 and UI-R-E1 library
                     in the form of single-stranded circles. The remaining
                     single-stranded circles (subtracted library) was purified
                     by hydroxyapatite column chromatography, converted to
                     double-stranded circles and electroporated into DH10B
                     bacteria (Life Technologies) to generate the UI-R-C0
                     library. This procedure has been previously described
                     (Bonaldo, Lennon and Soares, Genome Research 6: 791-806,
                     1996)"
ORIGIN      
        1 cggccgcaaa ggtttttttt ggacgttgta cagaggacat gactgctgaa gagcttcagc
       61 agttcttctg tcagtatgga gaagtggtag atgtcttcat tcccaaacca ttcagagctt
      121 ttgcctttgt tacctttgca gatgataagg ttgcccagtc tctttgtgga gaggacttga
      181 tcattaaagg aatcagcgtg catatatcca atgctgaacc taagcataat agcaatagac
      241 agttagaaag aagtggaaga tttggtggta atccaggtgg ctttgggaat cagggtgggt
      301 ttggtaacag tagagggggt ggagctggct tgggaaataa ccagggtggt aatatggatg
      361 gaaagcctcg tgccg
//
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