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GenBank: AA999071.1
FASTA Graphics
LOCUS AA999071 375 bp mRNA linear EST 26-JAN-2011 DEFINITION UI-R-C0-in-a-03-0-UI.s1 UI-R-C0 Rattus norvegicus cDNA clone UI-R-C0-in-a-03-0-UI 3', mRNA sequence. ACCESSION AA999071 VERSION AA999071.1 DBLINK BioSample: SAMN00155877 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 375) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT On Jun 5, 1998 this sequence version replaced gi:3189662. Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org Oligo-dT track not found, Not I site shown in beginning of sequence is likely internal to the message. cDNA Library Preparation: M. Fatima Bonaldo, Ph.D. Clone distribution: clones will be available through Research Genetics Seq primer: M13 Forward. FEATURES Location/Qualifiers source 1..375 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-C0-in-a-03-0-UI" /clone_lib="SAMN00155877 UI-R-C0" /dev_stage="adult" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; The UI-R-C0 library is a subtracted library derived from the UI-R-A1 and UI-R-E1 libraries. The UI-R-A1 library consisted of a mixture of individually tagged normalized libraries constructed from rat placenta, adult lung, brain, liver, kidney, heart, spleen, ovary, and muscle. The UI-R-E1 library consisted of a mixture of individually tagged normalized libraries constructed from 8, 12 and 18-day embryo. The tag is a string of 3-5 nucleotides present between the Not I site and the oligo-dT track which allows identification of the library of origin of a clone within the mixture. The subtracted library (UI-R-C0) was constructed as follows: PCR amplified cDNA inserts from a pool of UI-R-A1 and UI-R-E1 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the pooled UI-R-A1 and UI-R-E1 library in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (Life Technologies) to generate the UI-R-C0 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996)" ORIGIN 1 cggccgcaaa ggtttttttt ggacgttgta cagaggacat gactgctgaa gagcttcagc 61 agttcttctg tcagtatgga gaagtggtag atgtcttcat tcccaaacca ttcagagctt 121 ttgcctttgt tacctttgca gatgataagg ttgcccagtc tctttgtgga gaggacttga 181 tcattaaagg aatcagcgtg catatatcca atgctgaacc taagcataat agcaatagac 241 agttagaaag aagtggaaga tttggtggta atccaggtgg ctttgggaat cagggtgggt 301 ttggtaacag tagagggggt ggagctggct tgggaaataa ccagggtggt aatatggatg 361 gaaagcctcg tgccg //
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