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GenBank: AI188101.1
FASTA Graphics
LOCUS AI188101 494 bp mRNA linear EST 06-JAN-2011 DEFINITION qd65d01.x1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE:1734337 3', mRNA sequence. ACCESSION AI188101 VERSION AI188101.1 DBLINK BioSample: SAMN00155229 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 494) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima Bonaldo, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 1083 Std Error: 0.00 Seq primer: -40UP from Gibco High quality sequence stop: 434. FEATURES Location/Qualifiers source 1..494 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:1734337" /sex="male" /clone_lib="SAMN00155229 Soares_testis_NHT" /lab_host="DH10B" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was prepared from mRNA obtained from Clontech Laboratories, Inc., and primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCCAATTTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization to Cot5, and was constructed by Bento Soares and M. Fatima Bonaldo." ORIGIN 1 tttttttttt tttttttttt tttagcagcc cccacataat gactttattt gcttaatccc 61 caaattaaac gccgtttcaa gcgagggtcc tgtgttatta ctctcatcat gtcgagaaca 121 ttttcctgcc gagaccaatt tgaataaaac aaggggcctt ccttgaactc aatcaaggag 181 ccataatgtg gtgggtaata gaggttgctg atagatttgc aggcaaaagt gttataccta 241 atgatgggat atggataata gtcctcctgg aggtcctgat gcgattccag ttggctgcca 301 gatgcttggc tcgaatgcga aaattctcca ggtgtctctg acgtgattcc tttagtatct 361 tctttagtcg agtcttatgg caatataaca gccaagcaat ggcaatcaca cctgatgccc 421 acttctgctg gcgataaaag aggaagaatt ttcttgcttt gtagcatttc catgtggctt 481 ggatcttcat ggcg //
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