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nc05b08.y5 NCI_CGAP_Pr1 Homo sapiens cDNA clone IMAGE:1007223, mRNA sequence

GenBank: AI820948.1

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LOCUS       AI820948                 417 bp    mRNA    linear   EST 08-JAN-2011
DEFINITION  nc05b08.y5 NCI_CGAP_Pr1 Homo sapiens cDNA clone IMAGE:1007223, mRNA
            sequence.
ACCESSION   AI820948
VERSION     AI820948.1
DBLINK      BioSample: SAMN00155176
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 417)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Other_ESTs: nc05b08.x5
            Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: W. Marston Linehan, M.D., Rodrigo Chuaqui,
            M.D., Michael Emmert-Buck, M.D., Ph.D.
             cDNA Library Preparation: David B. Krizman, Ph.D.
             cDNA Library Arrayed by: Genome Systems Inc., Greg Lennon, Ph.D.
             DNA Sequencing by: Washington University Genome Sequencing Center
             Clone distribution: NCI-CGAP clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            www-bio.llnl.gov/bbrp/image/image.html
            
            This read is a RESEQUENCE of a previously sequenced human clone
            Original clone citation: WashU-NCI EST Project
            This read has been verified (found to hit its original self in the
            correct orientation)
            Putative full length read
            The vector to vector length is 418
            Insert Length: 549   Std Error: 0.00
            Seq primer: -40RP from Gibco.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:1007223"
                     /sex="male"
                     /clone_lib="SAMN00155176 NCI_CGAP_Pr1"
                     /dev_stage="45 years old"
                     /lab_host="DH10B"
                     /note="Vector: pAMP10; Site_1: Not1; Site_2: EcoRI; 1st
                     strand cDNA was primed with oligo(dT)17 on 50 ng of
                     DNAse-treated, total cellular RNA obtained from
                     5,000-10,000 microdissected, histologically normal
                     prostate epithelial cells. Double-stranded cDNA was
                     ligated to EcoRI adaptors, 5 cycles of PCR applied to the
                     cDNA with an adaptor-specific primer, and the resulting
                     PCR product subcloned into pAMP10 by the UDG-cloning
                     method (Life Technologies). Average insert size is 600 bp.
                     NOTE: Not directionally cloned. This library was
                     constructed by David Krizman."
ORIGIN      
        1 gtcgacacca gccccacctg acagaactgt ccgggggtac agtagagagt taaacattca
       61 ttggaaggag ctgaagaaaa gactatgtcc tttgagggga gcctagtttc agttaagcct
      121 ggaaggggaa aataaccatt ccttgaagag atagaggata tagagattac agtggaaagg
      181 tttggacaga gaagctgtag gaagctgatt ctagaaaggg aacatagaat ttggagatag
      241 agtatgggag aaggcagata acagatgact gaaaagctta ctaaattttg ttcttggatt
      301 gttcttaatg tgaatggtag ttacacattt tcaattgttt ttcttccaca gtcagtggga
      361 agacactata agataaatag aggttacaac ttaaagttgt atggttgcaa gttgtca
//
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