Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
GenBank: AI820948.1
FASTA Graphics
LOCUS AI820948 417 bp mRNA linear EST 08-JAN-2011 DEFINITION nc05b08.y5 NCI_CGAP_Pr1 Homo sapiens cDNA clone IMAGE:1007223, mRNA sequence. ACCESSION AI820948 VERSION AI820948.1 DBLINK BioSample: SAMN00155176 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 417) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Other_ESTs: nc05b08.x5 Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: W. Marston Linehan, M.D., Rodrigo Chuaqui, M.D., Michael Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: David B. Krizman, Ph.D. cDNA Library Arrayed by: Genome Systems Inc., Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html This read is a RESEQUENCE of a previously sequenced human clone Original clone citation: WashU-NCI EST Project This read has been verified (found to hit its original self in the correct orientation) Putative full length read The vector to vector length is 418 Insert Length: 549 Std Error: 0.00 Seq primer: -40RP from Gibco. FEATURES Location/Qualifiers source 1..417 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:1007223" /sex="male" /clone_lib="SAMN00155176 NCI_CGAP_Pr1" /dev_stage="45 years old" /lab_host="DH10B" /note="Vector: pAMP10; Site_1: Not1; Site_2: EcoRI; 1st strand cDNA was primed with oligo(dT)17 on 50 ng of DNAse-treated, total cellular RNA obtained from 5,000-10,000 microdissected, histologically normal prostate epithelial cells. Double-stranded cDNA was ligated to EcoRI adaptors, 5 cycles of PCR applied to the cDNA with an adaptor-specific primer, and the resulting PCR product subcloned into pAMP10 by the UDG-cloning method (Life Technologies). Average insert size is 600 bp. NOTE: Not directionally cloned. This library was constructed by David Krizman." ORIGIN 1 gtcgacacca gccccacctg acagaactgt ccgggggtac agtagagagt taaacattca 61 ttggaaggag ctgaagaaaa gactatgtcc tttgagggga gcctagtttc agttaagcct 121 ggaaggggaa aataaccatt ccttgaagag atagaggata tagagattac agtggaaagg 181 tttggacaga gaagctgtag gaagctgatt ctagaaaggg aacatagaat ttggagatag 241 agtatgggag aaggcagata acagatgact gaaaagctta ctaaattttg ttcttggatt 301 gttcttaatg tgaatggtag ttacacattt tcaattgttt ttcttccaca gtcagtggga 361 agacactata agataaatag aggttacaac ttaaagttgt atggttgcaa gttgtca //
Whole sequence Selected region from: to:
All features Gene, RNA, and CDS features only
Show reverse complement Show gap features
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on