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GenBank: AW125275.1
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LOCUS AW125275 302 bp mRNA linear EST 27-JAN-2011 DEFINITION UI-M-BH2.1-aps-h-09-0-UI.s1 NIH_BMAP_M_S3.1 Mus musculus cDNA clone UI-M-BH2.1-aps-h-09-0-UI 3', mRNA sequence. ACCESSION AW125275 VERSION AW125275.1 DBLINK BioSample: SAMN00157052 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 302) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Chin, H National Institute of Mental Health 6001 Executive Blvd. Room 7N-7190, MSC 9643, Bethesda, MD 20892-9643, USA Tel: 301 443 1706 Fax: 301 443 9890 Email: mEST@mail.nih.gov The sequence contained an oligo-dT track that was present in the oligonucleotide that was used to prime the synthesis of first strand cDNA and therefore this may represent a bonafide poly A tail. The sequence tag present in the cDNA between the NotI site and the oligo-dT track served to identify it as a clone from the normalized brain stems library cDNA Library Preparation: M.B. Soares Lab Clone distribution: NIH BMAP cDNA clones will be made available by the means that is soon to be determined. When NIH determines the means for distribution of the BMAP cDNA clones, this record will be updated accordingly when that means is determined. Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..302 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="taxon:10090" /clone="UI-M-BH2.1-aps-h-09-0-UI" /clone_lib="SAMN00157052 NIH_BMAP_M_S3.1" /dev_stage="27-32 days" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; The NIH_BMAP_M_S3.1 library is a subtracted library of a series, ultimately derived from a mixture of individually tagged normalized libraries from ten regions of the mouse brain (cerebellum, brain stems, olfactory bulbs, hypothalamus, cortex, amygdala, basal ganglia, pineal gland, striatum, hipoccampus) after a series of subtractions to reduce the representation of cDNAs from which ESTs had already been generated. The following serially subtracted libraries were generated in this process: NIH_BMAP_M_S3.1, NIH_BMAP_M_S2, NIH_BMAP_M_S1. The subtracted library (NIH_BMAP_M_S3.1) was constructed as follows: PCRamplified cDNA inserts from NIH_BMAP_M_S2 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the NIH_BMAP_M_S2 library in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (LifeTechnologies) to generate the NIH_BMAP_M_S3.1 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996); TAG_LIB=NIH_BMAP_M_S3.1; TAG_SEQ=TCATG; TAG_TISSUE=brain-stems" ORIGIN 1 tttttttttt tttttttctt gcaaaaacca atggtttatt aatcttatta gacagagcat 61 cttacatcta aatacactcc tcatgtacac agggtatcag tgattaatgg gctgtggctg 121 tcccggcaaa ctcacatgta cgtgctggct gtcccccaac ccctgacccc cgtgctccag 181 aggggactct ggtgctgctt cccagccggg ctagacattt ccagacttca attaaaaaga 241 ctttgcaatt gacaatttcc aggccatcct acatctcctc caaataaagc ggtggggtgg 301 gg //
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