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UI-M-BH2.1-aps-h-09-0-UI.s1 NIH_BMAP_M_S3.1 Mus musculus cDNA clone UI-M-BH2.1-aps-h-09-0-UI 3', mRNA sequence

GenBank: AW125275.1

FASTA Graphics 

LOCUS       AW125275                 302 bp    mRNA    linear   EST 27-JAN-2011
DEFINITION  UI-M-BH2.1-aps-h-09-0-UI.s1 NIH_BMAP_M_S3.1 Mus musculus cDNA clone
            UI-M-BH2.1-aps-h-09-0-UI 3', mRNA sequence.
ACCESSION   AW125275
VERSION     AW125275.1
DBLINK      BioSample: SAMN00157052
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Chin, H
            National Institute of Mental Health
            6001 Executive Blvd. Room 7N-7190, MSC 9643, Bethesda, MD
            20892-9643, USA
            Tel: 301 443 1706
            Fax: 301 443 9890
            Email: mEST@mail.nih.gov
            The sequence contained an oligo-dT track that was present in the
            oligonucleotide that was used to prime the synthesis of first
            strand cDNA and therefore this may represent a bonafide poly A
            tail. The sequence tag present in the cDNA between the NotI site
            and the oligo-dT track served to identify it as a clone from the
            normalized brain stems library cDNA Library Preparation: M.B.
            Soares Lab Clone distribution: NIH BMAP cDNA clones will be made
            available by the means that is soon to be determined. When NIH
            determines the means for distribution of the BMAP cDNA clones, this
            record will be updated accordingly when that means is determined.
            Seq primer: M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /clone="UI-M-BH2.1-aps-h-09-0-UI"
                     /clone_lib="SAMN00157052 NIH_BMAP_M_S3.1"
                     /dev_stage="27-32 days"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The NIH_BMAP_M_S3.1 library is a subtracted library of a
                     series, ultimately derived from a mixture of individually
                     tagged normalized libraries from ten regions of the mouse
                     brain (cerebellum, brain stems, olfactory bulbs,
                     hypothalamus, cortex, amygdala, basal ganglia, pineal
                     gland, striatum, hipoccampus) after a series of
                     subtractions to reduce the representation of cDNAs from
                     which ESTs had already been generated. The following
                     serially subtracted libraries were generated in this
                     process: NIH_BMAP_M_S3.1, NIH_BMAP_M_S2, NIH_BMAP_M_S1.
                     The subtracted library (NIH_BMAP_M_S3.1) was constructed
                     as follows: PCRamplified cDNA inserts from NIH_BMAP_M_S2
                     clones from which 3' ESTs had been derived was used as a
                     driver in a hybridization with the NIH_BMAP_M_S2 library
                     in the form of single-stranded circles. The remaining
                     single-stranded circles (subtracted library) was purified
                     by hydroxyapatite column chromatography, converted to
                     double-stranded circles and electroporated into DH10B
                     bacteria (LifeTechnologies) to generate the
                     NIH_BMAP_M_S3.1 library. This procedure has been
                     previously described (Bonaldo, Lennon and Soares, Genome
                     Research 6:791-806, 1996);
                     TAG_LIB=NIH_BMAP_M_S3.1;
                     TAG_SEQ=TCATG;
                     TAG_TISSUE=brain-stems"
ORIGIN      
        1 tttttttttt tttttttctt gcaaaaacca atggtttatt aatcttatta gacagagcat
       61 cttacatcta aatacactcc tcatgtacac agggtatcag tgattaatgg gctgtggctg
      121 tcccggcaaa ctcacatgta cgtgctggct gtcccccaac ccctgacccc cgtgctccag
      181 aggggactct ggtgctgctt cccagccggg ctagacattt ccagacttca attaaaaaga
      241 ctttgcaatt gacaatttcc aggccatcct acatctcctc caaataaagc ggtggggtgg
      301 gg
//
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