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GenBank: BE082843.1
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LOCUS BE082843 590 bp mRNA linear EST 12-MAY-2010 DEFINITION RC2-BT0642-210200-014-h10 BT0642 Homo sapiens cDNA, mRNA sequence. ACCESSION BE082843 VERSION BE082843.1 DBLINK BioSample: SAMN00159548 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 590) AUTHORS Dias Neto,E., Garcia Correa,R., Verjovski-Almeida,S., Briones,M.R., Nagai,M.A., da Silva,W. Jr., Zago,M.A., Bordin,S., Costa,F.F., Goldman,G.H., Carvalho,A.F., Matsukuma,A., Baia,G.S., Simpson,D.H., Brunstein,A., deOliveira,P.S., Bucher,P., Jongeneel,C.V., O'Hare,M.J., Soares,F., Brentani,R.R., Reis,L.F., de Souza,S.J. and Simpson,A.J. TITLE Shotgun sequencing of the human transcriptome with ORF expressed sequence tags JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3491-3496 (2000) PUBMED 10737800 COMMENT Contact: Simpson A.J.G. Laboratory of Cancer Genetics Ludwig Institute for Cancer Research Rua Prof. Antonio Prudente 109, 4 andar, 01509-010, Sao Paulo-SP, Brazil Tel: +55-11-2704922 Fax: +55-11-2707001 Email: asimpson@ludwig.org.br This sequence was derived from the FAPESP/LICR Human Cancer Genome Project. This entry can be seen in the following URL (http://www.ludwig.org.br/scripts/gethtml2.pl?t1=&t2=RC2-BT0642- 210200-014-h10&t3=2000-02-21&t4=1) Seq primer: puc 18 forward High quality sequence start: 39 High quality sequence stop: 583. FEATURES Location/Qualifiers source 1..590 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone_lib="SAMN00159548 BT0642" /dev_stage="Adult" /note="Organ: breast; Vector: puc18; Site_1: SmaI; Site_2: SmaI; A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions." ORIGIN 1 ccgtcctctc tgctcaagtt atagcataca tgtatcggga ttgtctttgg agcatgagat 61 tttcctccct caggagcttt tttttttcta ctcatgtcta actgcctact ctaacattct 121 cccctcaaca gattgagacc ctaaaaatct ttgagtttag gggcactgtg ggtcttccgt 181 aactgcttcc tgctgacatg ggataacgaa ctcatcagtg tctcattgtt ttgtcctttg 241 tcagggaaag atatcctcca tgttctctca ggtttgactt gcagtctcca gagaaaatgt 301 gtagggacac agttttccag cagggaaaaa agtaggttaa tattcctgtt agaggaccat 361 ttgaactctg agagtttcta ggtgagaagg acacaaatct ggttagtaga tttagtgagc 421 aaggcccaaa aaggagaaaa aggaagacaa aggccaggga tcccaacaaa atgagatgtc 481 atgaggtctg ggaccaggtg gcccccagga gactagacct aagtggctgt ttctgaaggg 541 aagaggctgg tgtataaatc ttcactgatc tggattgctt attgtaacca //
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