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GenBank: BE110877.1
FASTA Graphics
LOCUS BE110877 527 bp mRNA linear EST 27-JAN-2011 DEFINITION UI-R-BJ1-aux-a-07-0-UI.s1 UI-R-BJ1 Rattus norvegicus cDNA clone UI-R-BJ1-aux-a-07-0-UI 3', mRNA sequence. ACCESSION BE110877 VERSION BE110877.1 DBLINK BioSample: SAMN00158315 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 527) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org The sequence contained an oligo-dT track that was present in the oligonucleotide that was used to prime the synthesis of first strand cDNA and therefore this may represent a bonafide poly A tail. The sequence tag present in the cDNA between the NotI site and the oligo-dT track served to identify it as a clone from the normalized heart library cDNA Library Preparation: M.B. Soares Lab Clone distribution: clones will be available through Research Genetics (www.resgen.com) Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..527 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-BJ1-aux-a-07-0-UI" /clone_lib="SAMN00158315 UI-R-BJ1" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; The UI-R-BJ1 library is a subtracted library derived from the following tissues: atrium at 16.5 dpc, ventricle at 16.5 dpc, AV canal at 16.5 dpc, atrium at 15 dpc, ventricle at 15 dpc, AV canal at 15 dpc, ventricle at 13 dpc, and adult heart. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996); TAG_LIB=UI-R-BJ1; TAG_SEQ=ACAAC; TAG_TISSUE=heart" ORIGIN 1 tttttttttt tttttttcca aaaagagatt tttattagta aagataattt catactaaca 61 ttttatacac taaacgcgtc catccactgc aagagatatt aacatcttca cacaaagtgt 121 aaaacactct ttctccactc agtgtgctag ctctgtatac cttcacagca tcaataccag 181 gcagagttca tgacacaggg caccgaaagc ctgctctgcc ctcaggactg aaatgaaggg 241 gttcttctgg aagctggatg taaagaactt tgcagcgagt gctaagggaa cacaaggcag 301 atacagggac tctgtgctca ctgctgaaat agactgtcta atgctgtcac tgtcgctgtc 361 gacgagacat gtttggacag ctgacaagtg ggagagggca cccactcctg aatgggcttg 421 tggagtacac ttggggcact tagggaatac ttctagggga gcagctggag tgaaagttaa 481 catgggaaat gaaaatcaaa gtccatctgg gcaaacagta taaaagg //
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