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UI-R-BJ1-aux-a-07-0-UI.s1 UI-R-BJ1 Rattus norvegicus cDNA clone UI-R-BJ1-aux-a-07-0-UI 3', mRNA sequence

GenBank: BE110877.1

FASTA Graphics 

LOCUS       BE110877                 527 bp    mRNA    linear   EST 27-JAN-2011
DEFINITION  UI-R-BJ1-aux-a-07-0-UI.s1 UI-R-BJ1 Rattus norvegicus cDNA clone
            UI-R-BJ1-aux-a-07-0-UI 3', mRNA sequence.
ACCESSION   BE110877
VERSION     BE110877.1
DBLINK      BioSample: SAMN00158315
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            The sequence contained an oligo-dT track that was present in the
            oligonucleotide that was used to prime the synthesis of first
            strand cDNA and therefore this may represent a bonafide poly A
            tail. The sequence tag present in the cDNA between the NotI site
            and the oligo-dT track served to identify it as a clone from the
            normalized heart library cDNA Library Preparation: M.B. Soares Lab
            Clone distribution: clones will be available through Research
            Genetics (www.resgen.com)
            Seq primer: M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-BJ1-aux-a-07-0-UI"
                     /clone_lib="SAMN00158315 UI-R-BJ1"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-BJ1 library is a subtracted library derived from
                     the following tissues: atrium at 16.5 dpc, ventricle at
                     16.5 dpc, AV canal at 16.5 dpc, atrium at 15 dpc,
                     ventricle at 15 dpc, AV canal at 15 dpc, ventricle at 13
                     dpc, and adult heart. For a detailed description of the
                     library from which this clone was derived, please visit
                     our web site at ratest.eng.uiowa.edu. The subtraction has
                     been previously described in (Bonaldo, Lennon and Soares,
                     Genome Research 6:791-806, 1996);
                     TAG_LIB=UI-R-BJ1;
                     TAG_SEQ=ACAAC;
                     TAG_TISSUE=heart"
ORIGIN      
        1 tttttttttt tttttttcca aaaagagatt tttattagta aagataattt catactaaca
       61 ttttatacac taaacgcgtc catccactgc aagagatatt aacatcttca cacaaagtgt
      121 aaaacactct ttctccactc agtgtgctag ctctgtatac cttcacagca tcaataccag
      181 gcagagttca tgacacaggg caccgaaagc ctgctctgcc ctcaggactg aaatgaaggg
      241 gttcttctgg aagctggatg taaagaactt tgcagcgagt gctaagggaa cacaaggcag
      301 atacagggac tctgtgctca ctgctgaaat agactgtcta atgctgtcac tgtcgctgtc
      361 gacgagacat gtttggacag ctgacaagtg ggagagggca cccactcctg aatgggcttg
      421 tggagtacac ttggggcact tagggaatac ttctagggga gcagctggag tgaaagttaa
      481 catgggaaat gaaaatcaaa gtccatctgg gcaaacagta taaaagg
//
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