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GenBank: BF399817.1
FASTA Graphics
LOCUS BF399817 404 bp mRNA linear EST 27-JAN-2011 DEFINITION UI-R-CA1-bhz-b-11-0-UI.s1 UI-R-CA1 Rattus norvegicus cDNA clone UI-R-CA1-bhz-b-11-0-UI 3', mRNA sequence. ACCESSION BF399817 VERSION BF399817.1 DBLINK BioSample: SAMN00161733 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 404) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org The sequence contained an oligo-dT track that was present in the oligonucleotide that was used to prime the synthesis of first strand cDNA and therefore this may represent a bonafide poly A tail. The sequence tag present in the cDNA between the NotI site and the oligo-dT track served to identify it as a clone from the normalized corpus-striatum library cDNA Library Preparation: M.B. Soares Lab Clone distribution: clones will be available through Research Genetics (www.resgen.com) The following repetitive elements were found in this cDNA sequence: 2-92, >B1-F#SINE/Alu Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..404 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-CA1-bhz-b-11-0-UI" /clone_lib="SAMN00161733 UI-R-CA1" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; The UI-R-CA1 library is a subtracted library derived from the following tissues: thalamus, cerebellum, hypothalamus, medulla, pons, midbrain, cerebral cortex, corpus striatum, testis, and hippocampus. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996); TAG_LIB=UI-R-CA1; TAG_SEQ=CTAGG; TAG_TISSUE=corpus-striatum" ORIGIN 1 tttttttttt ttccttgaca agagtctcac tatgtagcct tggctgtcct agaactcatt 61 acatagacta ggttggcctc aaatacaggg atttaaggca tgcgacacca cacgtgtctt 121 ggaatcggcc tttgaatgaa cctcctgact ttcatcatga catcttgtga gaaaggcctg 181 tccttgactt gtcccatttc cacccacttc agggccatcc actccagggc cacagtccct 241 taccttggcg tgacccgcgt gagctcgtct gaagggtgca ggatgcggca ggtggtaaag 301 gtgaaagtcg agttggtctg tgacatgcac ttcccagggt ggtgcgctag atcaggagag 361 gaaggagaac aaacacattc tttgcgctca cataaaccat gaca //
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