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UI-R-CA1-bhz-b-11-0-UI.s1 UI-R-CA1 Rattus norvegicus cDNA clone UI-R-CA1-bhz-b-11-0-UI 3', mRNA sequence

GenBank: BF399817.1

FASTA Graphics 

LOCUS       BF399817                 404 bp    mRNA    linear   EST 27-JAN-2011
DEFINITION  UI-R-CA1-bhz-b-11-0-UI.s1 UI-R-CA1 Rattus norvegicus cDNA clone
            UI-R-CA1-bhz-b-11-0-UI 3', mRNA sequence.
ACCESSION   BF399817
VERSION     BF399817.1
DBLINK      BioSample: SAMN00161733
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            The sequence contained an oligo-dT track that was present in the
            oligonucleotide that was used to prime the synthesis of first
            strand cDNA and therefore this may represent a bonafide poly A
            tail. The sequence tag present in the cDNA between the NotI site
            and the oligo-dT track served to identify it as a clone from the
            normalized corpus-striatum library cDNA Library Preparation: M.B.
            Soares Lab Clone distribution: clones will be available through
            Research Genetics (www.resgen.com) The following repetitive
            elements were found in this cDNA sequence: 2-92, >B1-F#SINE/Alu
            Seq primer: M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-CA1-bhz-b-11-0-UI"
                     /clone_lib="SAMN00161733 UI-R-CA1"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-CA1 library is a subtracted library derived from
                     the following tissues: thalamus, cerebellum, hypothalamus,
                     medulla, pons, midbrain, cerebral cortex, corpus striatum,
                     testis, and hippocampus. For a detailed description of the
                     library from which this clone was derived, please visit
                     our web site at ratest.eng.uiowa.edu. The subtraction has
                     been previously described in (Bonaldo, Lennon and Soares,
                     Genome Research 6:791-806, 1996);
                     TAG_LIB=UI-R-CA1;
                     TAG_SEQ=CTAGG;
                     TAG_TISSUE=corpus-striatum"
ORIGIN      
        1 tttttttttt ttccttgaca agagtctcac tatgtagcct tggctgtcct agaactcatt
       61 acatagacta ggttggcctc aaatacaggg atttaaggca tgcgacacca cacgtgtctt
      121 ggaatcggcc tttgaatgaa cctcctgact ttcatcatga catcttgtga gaaaggcctg
      181 tccttgactt gtcccatttc cacccacttc agggccatcc actccagggc cacagtccct
      241 taccttggcg tgacccgcgt gagctcgtct gaagggtgca ggatgcggca ggtggtaaag
      301 gtgaaagtcg agttggtctg tgacatgcac ttcccagggt ggtgcgctag atcaggagag
      361 gaaggagaac aaacacattc tttgcgctca cataaaccat gaca
//
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