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GenBank: BF405339.1
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LOCUS BF405339 517 bp mRNA linear EST 26-JAN-2011 DEFINITION UI-R-CA1-bit-a-04-0-UI.s1 UI-R-CA1 Rattus norvegicus cDNA clone UI-R-CA1-bit-a-04-0-UI 3', mRNA sequence. ACCESSION BF405339 VERSION BF405339.1 DBLINK BioSample: SAMN00161733 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 517) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org The sequence contained an oligo-dT track that was present in the oligonucleotide that was used to prime the synthesis of first strand cDNA and therefore this may represent a bonafide poly A tail. The sequence tag present in the cDNA between the NotI site and the oligo-dT track served to identify it as a clone from the normalized corpus-striatum library cDNA Library Preparation: M.B. Soares Lab Clone distribution: clones will be available through Research Genetics (www.resgen.com) Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..517 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-CA1-bit-a-04-0-UI" /clone_lib="SAMN00161733 UI-R-CA1" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; The UI-R-CA1 library is a subtracted library derived from the following tissues: thalamus, cerebellum, hypothalamus, medulla, pons, midbrain, cerebral cortex, corpus striatum, testis, and hippocampus. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996); TAG_LIB=UI-R-CA1; TAG_SEQ=CTAGG; TAG_TISSUE=corpus-striatum" ORIGIN 1 tttttttttt tttttttaag tttgaggagt ttccacagat accatcataa caacataaga 61 ttatttgtaa atcagctctg gctttcttta gagcaatgtg ctgaatgcta aggattggag 121 gctgagattg tgtgttctct cttctttcaa agacagctga tttgcctcag aaagtgctta 181 aaacatagct ctcctttgag atcctcagag agcaagcctg tgctctccaa gacaccagtt 241 attcaagcaa gaagacagtc aaagctattt tccatctgtg atggcgaaag tcattcatca 301 ttaaataaag catttaagca gtaatttaaa tattacaggc tatgaaactg cttaggcagg 361 gaacaagaac cagaattaga agaccaggct tgtccttgag caaataatta aaacctagct 421 gtttctcaca cttgtcttta aaaaaaatgg aatgaaaatt ctagtcattc atccatttca 481 ctccatttta gctcatagaa ccaatgaata gtcacga //
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