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GenBank: BF415023.1
FASTA Graphics
LOCUS BF415023 573 bp mRNA linear EST 28-JAN-2011 DEFINITION UI-R-BJ2-boy-g-07-0-UI.s1 UI-R-BJ2 Rattus norvegicus cDNA clone UI-R-BJ2-boy-g-07-0-UI 3', mRNA sequence. ACCESSION BF415023 VERSION BF415023.1 DBLINK BioSample: SAMN00161736 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 573) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org The sequence contained an oligo-dT track that was present in the oligonucleotide that was used to prime the synthesis of first strand cDNA and therefore this may represent a bonafide poly A tail. cDNA Library Preparation: M.B. Soares Lab Clone distribution: clones will be available through Research Genetics (www.resgen.com) Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..573 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-BJ2-boy-g-07-0-UI" /clone_lib="SAMN00161736 UI-R-BJ2" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; The UI-R-BJ2 library is a subtracted library derived from the following tissues: heart, atrium at 15 dpc, ventricle at 16.5 dpc, atrium at 16.5 dpc, ventricle at 13 dpc, ventricle at 15 dpc, AV canal at 15 dpc. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996); TAG_SEQ=None found" ORIGIN 1 tttttttttt ttttttttcc taccttcagt tctttataga atatgatcct tttgtagaac 61 aaaagttaca ccagtgacaa ttatttacag atcccaaaca gactcaagct ttacacacac 121 agaattgaac attctaggtc agtgattagt cagcaattaa acacctgccc agatcacaca 181 gttcagtaca aaccccagtg tctctgtaac tatgactaga ggctgcctag gcccctggcc 241 cagccctgtc cccctgagct ccacccaaag acctgctggt ggccaatggt ggaactaaca 301 gtggaacttg gggctgccac cgtcacctcc ctgacccact gccctggcct atggtcagtc 361 ttcatctctc tttcccagag aaagagactc aaactaaacc acttactcca ccccgaaagc 421 ctccacagcc taaggcctct ctgtggcacg ataggtggca gcgctcacta acccgggcac 481 tgccagggtc cataaagcgt taaccactgt gtgtggctat cacctgtgaa gccatcccca 541 cagacgggcc atgtcgcccc ccctctggca tgt //
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