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UI-R-C1-lj-d-06-0-UI.r1 UI-R-C1 Rattus norvegicus cDNA clone UI-R-C1-lj-d-06-0-UI 5', mRNA sequence

GenBank: BF549571.1

FASTA Graphics 

LOCUS       BF549571                 445 bp    mRNA    linear   EST 26-JAN-2011
DEFINITION  UI-R-C1-lj-d-06-0-UI.r1 UI-R-C1 Rattus norvegicus cDNA clone
            UI-R-C1-lj-d-06-0-UI 5', mRNA sequence.
ACCESSION   BF549571
VERSION     BF549571.1
DBLINK      BioSample: SAMN00155931
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            cDNA Library Preparation: M.B. Soares Lab Clone distribution:
            clones will be available through Research Genetics (www.resgen.com)
            This clone is also available through the I.M.A.G.E. Consortium at
            LLNL (info@image.llnl.gov). IMAGE ID= 1775891 The following
            repetitive elements were found in this cDNA sequence: 393-414,
            >AT_rich#Low_complexity
            Seq primer: M13 Forward.
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-C1-lj-d-06-0-UI"
                     /clone_lib="SAMN00155931 UI-R-C1"
                     /dev_stage="Adult"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-C1 library is a subtracted library derived from
                     the UI-R-C0 library, which is a subtracted library derived
                     from the UI-R-A1 and UI-R-E1 libraries. The UI-R-A1
                     library consisted of a mixture of individually tagged
                     normalized libraries constructed from rat placenta, adult
                     lung, brain, liver, kidney, heart, spleen, ovary, and
                     muscle. The UI-R-E1 library consisted of a mixture of
                     individually tagged normalized libraries constructed from
                     8, 12 and 18-day embryo. The tag is a string of 3-5
                     nucleotides present between the Not I site and the
                     oligo-dT track which allows identification of the library
                     of origin of a clone within the mixture. The subtracted
                     library (UI-R-C1) was constructed as follows: PCR
                     amplified cDNA inserts from UI-R-C0 clones from which 3'
                     ESTs had been derived was used as a driver in a
                     hybridization with the UI-R-C0 library in the form of
                     single-stranded circles. The remaining single-stranded
                     circles (subtracted library) was purified by
                     hydroxyapatite column chromatography, converted to
                     double-stranded circles and electroporated into DH10B
                     bacteria (Life Technologies) to generate the UI-R-C1
                     library. This procedure has been previously described
                     (Bonaldo, Lennon and Soares, Genome Research 6: 791-806,
                     1996)"
ORIGIN      
        1 cacgagggtc gcctctggac caatggtccc atcgctgaag tgctccagca gggcctgcag
       61 cccatcgtga gccatgccac gtgctccagg ttggactggt ggttcatcaa ggtccggaag
      121 acgatggtgt gcgctggggg tgatggcgtc atctctgcct gtaacggaga ttctggcggc
      181 ccactgaact gccaagcaga agacggctca tggcaggtgc acggcatcgt gagcttcggt
      241 tccagtagcg gctgcaacgt acacaagaaa ccggtagtct tcacccgagt gtctgcctac
      301 aatgactgga tcaacgagaa aatacaactg tgaagaggcc gttccagaag tctcgctgcn
      361 cgtctcccaa cagcaataaa gcttcttcca tgataaaaaa aaaaaaaaaa aaatctcgcg
      421 gccgcaagct tattcccttt agtga
//
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