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GenBank: BI076805.1
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LOCUS BI076805 544 bp mRNA linear EST 12-MAY-2010 DEFINITION L0265A12-3 NIA Mouse Newborn Ovary cDNA Library Mus musculus cDNA clone L0265A12 3', mRNA sequence. ACCESSION BI076805 VERSION BI076805.1 DBLINK BioSample: SAMN00158334 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 544) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: L0265 row: A column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..544 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:L0265A12-3" /db_xref="taxon:10090" /clone="L0265A12" /sex="female" /clone_lib="SAMN00158334 NIA Mouse Newborn Ovary cDNA Library" /dev_stage="Newborn Ovary" /lab_host="DH10B" /note="Vector: pSPORT1 (Gibco/BRL Life Technology); Site_1: SalI; Site_2: NotI; Total RNAs were extracted from 7 Newborn Ovary. The double-stranded cDNA was synthesized by Gibco's kit with an Oligo(dT) primer [NotI primer-adapter from GibcoBRL] [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.56ug of total RNA . The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (include Sal1 sequence). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high fidelity PCR using Takara's Ex Taq polymerase. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were digested with SalI and NotI enzymes. Then, the cDNAs were size selected by Gibco's Size Fractionation Column. The cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical method. The library was constructed by Xiaohong Wang and Yulan Piao." ORIGIN 1 ctctatatat ctcccttgtc aataccaccc ctgtttagat ctgccttggg ccgaaatccc 61 gggaagaaaa atcctcgctg gaataataat ccgggatcta acaagggcgg ctcaccccca 121 tagactctca gttcccatcc acgcacagca ggccacgcca tcaagggggg caagaagctc 181 cagcgggtca aggcagcaag gacaacccag ggggaaaatg gccccagggg gatccaggaa 241 caccagcgga gagggcaaga tgagtgtgtc tgaggatcag gaagtaaagt gcaagccata 301 tgagttagct gcagtcactg gggcccaaag gactgccgcc acacgcgatc cccacaattc 361 ccccgtttga cattcgtggc agatacaagg tctggagaca gcccctcgaa agcatgggcc 421 ttacccgacc gtgggagaca aacaggattg gaatgatccc aagtccaagg ttggggaaat 481 gcccagggag tctcatcact gacgacctct atatcaggcc aagccagtcc agcggagatt 541 gtgg //
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