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L0265A12-3 NIA Mouse Newborn Ovary cDNA Library Mus musculus cDNA clone L0265A12 3', mRNA sequence

GenBank: BI076805.1

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LOCUS       BI076805                 544 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  L0265A12-3 NIA Mouse Newborn Ovary cDNA Library Mus musculus cDNA
            clone L0265A12 3', mRNA sequence.
ACCESSION   BI076805
VERSION     BI076805.1
DBLINK      BioSample: SAMN00158334
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            Plate: L0265  row: A  column: 12
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:L0265A12-3"
                     /db_xref="taxon:10090"
                     /clone="L0265A12"
                     /sex="female"
                     /clone_lib="SAMN00158334 NIA Mouse Newborn Ovary cDNA
                     Library"
                     /dev_stage="Newborn Ovary"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1 (Gibco/BRL Life Technology);
                     Site_1: SalI; Site_2: NotI; Total RNAs were extracted from
                     7 Newborn Ovary. The double-stranded cDNA was synthesized
                     by Gibco's kit with an Oligo(dT) primer [NotI
                     primer-adapter from GibcoBRL]
                     [5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from
                     2.56ug of total RNA . The double-stranded cDNAs were
                     treated with T4 DNA polymerase and purified by
                     ethanol-precipitation. The cDNAs were ligated to
                     Lone-linker LL-Sal3 (include Sal1 sequence). The cDNAs
                     were purified by phenol/chloroform and separated from free
                     linkers by Centricon 100. Then, cDNAs were amplified by
                     long-range high fidelity PCR using Takara's Ex Taq
                     polymerase. Then, the cDNAs were purified by
                     phenol/chloroform and by Centricon 100. The cDNAs were
                     digested with SalI and NotI enzymes. Then, the cDNAs were
                     size selected by Gibco's Size Fractionation Column. The
                     cDNAs were cloned into SalI/NotI site of pSPORT1 plasmid
                     vector. The DH10B E. coli host was transformed with the
                     ligation mixture by chemical method. The library was
                     constructed by Xiaohong Wang and Yulan Piao."
ORIGIN      
        1 ctctatatat ctcccttgtc aataccaccc ctgtttagat ctgccttggg ccgaaatccc
       61 gggaagaaaa atcctcgctg gaataataat ccgggatcta acaagggcgg ctcaccccca
      121 tagactctca gttcccatcc acgcacagca ggccacgcca tcaagggggg caagaagctc
      181 cagcgggtca aggcagcaag gacaacccag ggggaaaatg gccccagggg gatccaggaa
      241 caccagcgga gagggcaaga tgagtgtgtc tgaggatcag gaagtaaagt gcaagccata
      301 tgagttagct gcagtcactg gggcccaaag gactgccgcc acacgcgatc cccacaattc
      361 ccccgtttga cattcgtggc agatacaagg tctggagaca gcccctcgaa agcatgggcc
      421 ttacccgacc gtgggagaca aacaggattg gaatgatccc aagtccaagg ttggggaaat
      481 gcccagggag tctcatcact gacgacctct atatcaggcc aagccagtcc agcggagatt
      541 gtgg
//
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