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GenBank: BI292508.1
FASTA Graphics
LOCUS BI292508 438 bp mRNA linear EST 26-JAN-2011 DEFINITION UI-R-DO0-ciw-i-23-0-UI.s1 UI-R-DO0 Rattus norvegicus cDNA clone UI-R-DO0-ciw-i-23-0-UI 3', mRNA sequence. ACCESSION BI292508 VERSION BI292508.1 DBLINK BioSample: SAMN00164157 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 438) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org The sequence contained an oligo-dT track that was present in the oligonucleotide that was used to prime the synthesis of first strand cDNA and therefore this may represent a bonafide poly A tail. The sequence tag present in the cDNA between the NotI site and the oligo-dT track served to verify it as a clone from the non-normalized rat cell line R3327-5p library cDNA Library Preparation: M.B. Soares Lab Clone distribution: clones will be available through Research Genetics (www.resgen.com) Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..438 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-DO0-ciw-i-23-0-UI" /clone_lib="SAMN00164157 UI-R-DO0" /dev_stage="ADULT" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; The UI-R-DO0 library is a non-normalized Rat Cell Line R3327-5P library constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag CCAGG between the Not I cloning site and dT18 stretch. TAG_LIB=UI-R-DO0; TAG_SEQ=CACGTGAGAT; TAG_TISSUE=rat cell line R3327-5p" ORIGIN 1 tttttttttt ttttttttcc cattatgcac acgggttact gagatgtttt attcattcac 61 ttaaccatgc ccaacacatc gcacagcaga tgtctttgga gacgggaatg gctgctctgg 121 ccacccagct gcttcagaag tatgacaaat aagcaaactc aactttattc atgagatggg 181 gaggggaggt ttgggaatcg ctgcctttac attagcatta caatcgcgga tgccctgaca 241 gatgctatca gacaaaagga gagagcaaag ggagagagag accagacaca gaggatgctc 301 ctcctccaaa acccaatgca cttgttagac ttaattccaa agtctcaatc gaaagaagag 361 gcaacagcga acactttaat atgtagagaa catcactgcc tccaggaatc gctctccagc 421 ctcacaggtc tggagcac //
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