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UI-R-DO0-ciw-i-23-0-UI.s1 UI-R-DO0 Rattus norvegicus cDNA clone UI-R-DO0-ciw-i-23-0-UI 3', mRNA sequence

GenBank: BI292508.1

FASTA Graphics 

LOCUS       BI292508                 438 bp    mRNA    linear   EST 26-JAN-2011
DEFINITION  UI-R-DO0-ciw-i-23-0-UI.s1 UI-R-DO0 Rattus norvegicus cDNA clone
            UI-R-DO0-ciw-i-23-0-UI 3', mRNA sequence.
ACCESSION   BI292508
VERSION     BI292508.1
DBLINK      BioSample: SAMN00164157
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            The sequence contained an oligo-dT track that was present in the
            oligonucleotide that was used to prime the synthesis of first
            strand cDNA and therefore this may represent a bonafide poly A
            tail. The sequence tag present in the cDNA between the NotI site
            and the oligo-dT track served to verify it as a clone from the
            non-normalized rat cell line R3327-5p library cDNA Library
            Preparation: M.B. Soares Lab Clone distribution: clones will be
            available through Research Genetics (www.resgen.com)
            Seq primer: M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-DO0-ciw-i-23-0-UI"
                     /clone_lib="SAMN00164157 UI-R-DO0"
                     /dev_stage="ADULT"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-DO0 library is a non-normalized Rat Cell Line
                     R3327-5P library constructed in pT3T7 PAC vector according
                     to the procedure described by Bonaldo, Lennon & Soares
                     (Genome Research Genome 6: 791-806, 1996). The
                     oligonucleotide used to prime first strand synthesis
                     contained the sequence tag CCAGG between the Not I cloning
                     site and dT18 stretch.
                     TAG_LIB=UI-R-DO0;
                     TAG_SEQ=CACGTGAGAT;
                     TAG_TISSUE=rat cell line R3327-5p"
ORIGIN      
        1 tttttttttt ttttttttcc cattatgcac acgggttact gagatgtttt attcattcac
       61 ttaaccatgc ccaacacatc gcacagcaga tgtctttgga gacgggaatg gctgctctgg
      121 ccacccagct gcttcagaag tatgacaaat aagcaaactc aactttattc atgagatggg
      181 gaggggaggt ttgggaatcg ctgcctttac attagcatta caatcgcgga tgccctgaca
      241 gatgctatca gacaaaagga gagagcaaag ggagagagag accagacaca gaggatgctc
      301 ctcctccaaa acccaatgca cttgttagac ttaattccaa agtctcaatc gaaagaagag
      361 gcaacagcga acactttaat atgtagagaa catcactgcc tccaggaatc gctctccagc
      421 ctcacaggtc tggagcac
//
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