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GenBank: BQ193179.1
FASTA Graphics
LOCUS BQ193179 587 bp mRNA linear EST 28-JAN-2011 DEFINITION UI-R-DR1-clk-n-22-0-UI.s1 UI-R-DR1 Rattus norvegicus cDNA clone UI-R-DR1-clk-n-22-0-UI 3', mRNA sequence. ACCESSION BQ193179 VERSION BQ193179.1 DBLINK BioSample: SAMN00170068 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 587) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org The sequence contained an oligo-dT track that was present in the oligonucleotide that was used to prime the synthesis of first strand cDNA and therefore this may represent a bonafide poly A tail. The sequence tag present in the cDNA between the NotI site and the oligo-dT track served to verify it as a clone from the normalized osteoblast library cDNA Library Preparation: M.B. Soares Lab Clone distribution: clones will be available through Research Genetics (www.resgen.com) The following repetitive elements were found in this cDNA sequence: 1-43, >AT_rich#Low_complexity 224-245, >AT_rich#Low_complexity Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..587 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-DR1-clk-n-22-0-UI" /clone_lib="SAMN00170068 UI-R-DR1" /dev_stage="adult" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; The UI-R-DR1 library is a normalized Rat Osteoblast library (nREO) constructed in pT3T7 vector according to the procedure described by Bonaldo, Lennon & Soares (Normalization and Subtraction: Two Approaches to Facilitate Gene Discovery. Genome Research 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag AAGATATCAA between the Not I cloning site and dT18 stretch. The Rat Osteoblast tissue was provided by Lian & Stein of the University of Massachussetts Medical School. TAG_LIB=UI-R-DR1; TAG_SEQ=AAGATATCAA; TAG_TISSUE=osteoblast" ORIGIN 1 tttttttttt ttttttttaa aagttgaatt tttttttaaa aaagcacaaa acccctacat 61 actataatga tacacatcaa gtattgatcc agtcttacat cgacctattg acgattacca 121 gcaccatgtt ttttctcacc taggttatta acctattgac aatttccagc acctaggttt 181 ttctcaccac gcctggagga tcgttattca aagtatatgt tcctttaaaa ttaaaaaaaa 241 aaaaacccaa aaaacaaaaa ccttttagga caaactctca ggaaacaaac aagacctttt 301 aaaacaatca ggttattcat gacaccatca aatttccttt agaaaaataa taggtttttt 361 aaacacttta ccaactgatg gaatctgaga tactgttatt cagtgtgaca aatgaaccga 421 cctgctgatg gtgggggatg ccctggagga ccttagagcc tccacgtgga aagtgagcgc 481 cctccttggc tgctaaagct gtgcacttct gaatttagac caaggtccca tcagatctaa 541 attaggtaca gtcacaactt taaatgatga acagaattag ggaaatg //
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