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UI-R-DR1-clk-n-22-0-UI.s1 UI-R-DR1 Rattus norvegicus cDNA clone UI-R-DR1-clk-n-22-0-UI 3', mRNA sequence

GenBank: BQ193179.1

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LOCUS       BQ193179                 587 bp    mRNA    linear   EST 28-JAN-2011
DEFINITION  UI-R-DR1-clk-n-22-0-UI.s1 UI-R-DR1 Rattus norvegicus cDNA clone
            UI-R-DR1-clk-n-22-0-UI 3', mRNA sequence.
ACCESSION   BQ193179
VERSION     BQ193179.1
DBLINK      BioSample: SAMN00170068
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            The sequence contained an oligo-dT track that was present in the
            oligonucleotide that was used to prime the synthesis of first
            strand cDNA and therefore this may represent a bonafide poly A
            tail. The sequence tag present in the cDNA between the NotI site
            and the oligo-dT track served to verify it as a clone from the
            normalized osteoblast library cDNA Library Preparation: M.B. Soares
            Lab Clone distribution: clones will be available through Research
            Genetics (www.resgen.com) The following repetitive elements were
            found in this cDNA sequence: 1-43, >AT_rich#Low_complexity 224-245,
            >AT_rich#Low_complexity
            Seq primer: M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-DR1-clk-n-22-0-UI"
                     /clone_lib="SAMN00170068 UI-R-DR1"
                     /dev_stage="adult"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-DR1 library is a normalized Rat Osteoblast
                     library (nREO) constructed in pT3T7 vector according to
                     the procedure described by Bonaldo, Lennon & Soares
                     (Normalization and Subtraction: Two Approaches to
                     Facilitate Gene Discovery. Genome Research 6: 791-806,
                     1996). The oligonucleotide used to prime first strand
                     synthesis contained the sequence tag AAGATATCAA between
                     the Not I cloning site and dT18 stretch. The Rat
                     Osteoblast tissue was provided by Lian & Stein of the
                     University of Massachussetts Medical School.
                     TAG_LIB=UI-R-DR1;
                     TAG_SEQ=AAGATATCAA;
                     TAG_TISSUE=osteoblast"
ORIGIN      
        1 tttttttttt ttttttttaa aagttgaatt tttttttaaa aaagcacaaa acccctacat
       61 actataatga tacacatcaa gtattgatcc agtcttacat cgacctattg acgattacca
      121 gcaccatgtt ttttctcacc taggttatta acctattgac aatttccagc acctaggttt
      181 ttctcaccac gcctggagga tcgttattca aagtatatgt tcctttaaaa ttaaaaaaaa
      241 aaaaacccaa aaaacaaaaa ccttttagga caaactctca ggaaacaaac aagacctttt
      301 aaaacaatca ggttattcat gacaccatca aatttccttt agaaaaataa taggtttttt
      361 aaacacttta ccaactgatg gaatctgaga tactgttatt cagtgtgaca aatgaaccga
      421 cctgctgatg gtgggggatg ccctggagga ccttagagcc tccacgtgga aagtgagcgc
      481 cctccttggc tgctaaagct gtgcacttct gaatttagac caaggtccca tcagatctaa
      541 attaggtaca gtcacaactt taaatgatga acagaattag ggaaatg
//
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