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UI-R-FF0-cov-o-16-0-UI.s1 NCI_CGAP_FF0 Rattus norvegicus cDNA clone UI-R-FF0-cov-o-16-0-UI 3', mRNA sequence

GenBank: BQ779753.1

FASTA Graphics 

LOCUS       BQ779753                 448 bp    mRNA    linear   EST 26-JAN-2011
DEFINITION  UI-R-FF0-cov-o-16-0-UI.s1 NCI_CGAP_FF0 Rattus norvegicus cDNA clone
            UI-R-FF0-cov-o-16-0-UI 3', mRNA sequence.
ACCESSION   BQ779753
VERSION     BQ779753.1
DBLINK      BioSample: SAMN00170794
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            Tissue Procurement: Jeff Stevens
             cDNA Library preparation: Dr. M. Bento Soares, University of Iowa
             cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa
             DNA Sequencing by: Dr. M. Bento Soares, University of Iowa
             Clone Distribution: DISTRIBUTION: Researchers may obtain clones
            from Research Genetics (www.resgen.com).
             The following repetitive elements were found in this cDNA
            sequence: 21-167, >B3A#SINE/B2 (matched compliment) 357-441,
            >ID_RN#SINE/ID
            Seq primer: M13 FORWARD
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /clone="UI-R-FF0-cov-o-16-0-UI"
                     /tissue_type="Mixed tissues"
                     /clone_lib="SAMN00170794 NCI_CGAP_FF0"
                     /dev_stage="Adult"
                     /lab_host="DH10B (Life Technologies) (T1 phage resistant)"
                     /note="Vector: pT7T3-Pac (Pharmacia) with a modified
                     polylinker; Site_1: EcoR I; Site_2: Not I; UI-R-FF0 is a
                     subtracted cDNA library containing the following
                     tissue(s): Normal cartilage and SR-JWS Tumor Line . The
                     subtraction was made according to Bonaldo, Lennon and
                     Soares, Genome Research, 6:791-806, 1996. The
                     oligonucleotide used to prime the synthesis of
                     first-strand cDNA contains a library tag sequence that is
                     located between the Not I site and the (dT)18 tail. The
                     sequence tags for these libraries are: CTAATGGACG,
                     CATTCTTGTA.
                     TAG_LIB=UI-R-FF0;
                     TAG_SEQ=CTAATGGACG;
                     TAG_TISSUE=cartilage"
ORIGIN      
        1 tttttttttt ttttttcact tgtgtgtgcc cgtgagtgcc cagagggcat gggatccact
       61 agggctggag ctacagctga gtgtaaggca cctgactctg gagtcacagt cctgtctcca
      121 gcaagagcac caagcaccgt taactgctga gccatctccc ccgctccagc acctttggtt
      181 attgatattt gctttgtaaa ttaccagaga ataagaatgg ctttattttt ttctccagtc
      241 ttatgaattt aacacatata taaatgcaca tagacaagtt acagaaaaac tcccatcttg
      301 tcccaggtta tgttgttgtt gttaatttcc ttttcttaaa aaacaggttc gtagggggct
      361 ggggatttag ctcagtggta gagcgcttac ctaggaagcg caaggccctg ggttcagtcc
      421 ccagttccgg aaaaaaaaac ctcgtgcc
//
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