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GenBank: BQ779753.1
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LOCUS BQ779753 448 bp mRNA linear EST 26-JAN-2011 DEFINITION UI-R-FF0-cov-o-16-0-UI.s1 NCI_CGAP_FF0 Rattus norvegicus cDNA clone UI-R-FF0-cov-o-16-0-UI 3', mRNA sequence. ACCESSION BQ779753 VERSION BQ779753.1 DBLINK BioSample: SAMN00170794 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 448) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org Tissue Procurement: Jeff Stevens cDNA Library preparation: Dr. M. Bento Soares, University of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa DNA Sequencing by: Dr. M. Bento Soares, University of Iowa Clone Distribution: DISTRIBUTION: Researchers may obtain clones from Research Genetics (www.resgen.com). The following repetitive elements were found in this cDNA sequence: 21-167, >B3A#SINE/B2 (matched compliment) 357-441, >ID_RN#SINE/ID Seq primer: M13 FORWARD POLYA=Yes. FEATURES Location/Qualifiers source 1..448 /organism="Rattus norvegicus" /mol_type="mRNA" /db_xref="taxon:10116" /clone="UI-R-FF0-cov-o-16-0-UI" /tissue_type="Mixed tissues" /clone_lib="SAMN00170794 NCI_CGAP_FF0" /dev_stage="Adult" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /note="Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; UI-R-FF0 is a subtracted cDNA library containing the following tissue(s): Normal cartilage and SR-JWS Tumor Line . The subtraction was made according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tags for these libraries are: CTAATGGACG, CATTCTTGTA. TAG_LIB=UI-R-FF0; TAG_SEQ=CTAATGGACG; TAG_TISSUE=cartilage" ORIGIN 1 tttttttttt ttttttcact tgtgtgtgcc cgtgagtgcc cagagggcat gggatccact 61 agggctggag ctacagctga gtgtaaggca cctgactctg gagtcacagt cctgtctcca 121 gcaagagcac caagcaccgt taactgctga gccatctccc ccgctccagc acctttggtt 181 attgatattt gctttgtaaa ttaccagaga ataagaatgg ctttattttt ttctccagtc 241 ttatgaattt aacacatata taaatgcaca tagacaagtt acagaaaaac tcccatcttg 301 tcccaggtta tgttgttgtt gttaatttcc ttttcttaaa aaacaggttc gtagggggct 361 ggggatttag ctcagtggta gagcgcttac ctaggaagcg caaggccctg ggttcagtcc 421 ccagttccgg aaaaaaaaac ctcgtgcc //
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