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GenBank: CA505718.1
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LOCUS CA505718 709 bp mRNA linear EST 27-JAN-2011 DEFINITION UI-R-FJ0-cpz-e-08-0-UI.s1 UI-R-FJ0 Rattus norvegicus cDNA clone UI-R-FJ0-cpz-e-08-0-UI 3', mRNA sequence. ACCESSION CA505718 VERSION CA505718.1 DBLINK BioSample: SAMN00171765 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 709) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org Tissue Procurement: Dr. James Lin, Universtiy of Iowa cDNA Library preparation: Dr. M. Bento Soares, University of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa DNA Sequencing by: Dr. M. Bento Soares, University of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 FORWARD POLYA=Yes. FEATURES Location/Qualifiers source 1..709 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-FJ0-cpz-e-08-0-UI" /tissue_type="embryo" /clone_lib="SAMN00171765 UI-R-FJ0" /dev_stage="embryo" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /note="Vector: pYX-Asc; Site_1: EcoR I; Site_2: Not I; UI-R-FJ0 is a cDNA library containing the following tissue(s): rat embryo. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CATCTCTACT. This library was created for the University of Iowa Program for Rat Gene Discovery and Mapping (Val Sheffield, Bento Soares and Tom Casavant); TAG_LIB=UI-R-FJ0; TAG_SEQ=CATCTCTACT; TAG_TISSUE=rat-embryo" ORIGIN 1 tttttttttt ttttttttgg tggggggagg ggtgttgttc ttttggttta ttttatccta 61 tctcaaccta ttacatgggt ttgcaaagga agcccctttc tgcctcccta gctataggta 121 gaaaagtctg gctgatgact attttgtctg agctgtgata ctattttaaa aggatgggcg 181 ggagaggttc ataaggagag cagggaagtc cagagatagg ttgacaggga aggggactaa 241 gttgcatgga ggtatgaagt aaacacaggc atagaaatga aaaatctgtc ctcatcagca 301 tcccttcttg tcccttctaa gcaaggtata aggaataagg gaaatgacat agatacccgg 361 ttgtggccca aagccttacc cttctcaggg ctcacaacat gatagcaata agcatatgtt 421 cacaaatagc aggctgcccg ccagtctgac aaccagtagg tttcctaaag tctggggagg 481 ctaggcttgt agcccatgca ctgagaagat gcctgggtcc gtaggcagtt cttgaggtct 541 ttaattggag ccaataacat tgacctagcc aaaagtcaat ggactgagtg caaccaggca 601 cgaccagact tgatgagggt tcactagagg actgtctccg aggcaagagg agacatacca 661 tgtgaacana acatactcct gtttgccctt gtcctcatcc acacccagt //
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