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UI-R-FJ0-cpz-e-08-0-UI.s1 UI-R-FJ0 Rattus norvegicus cDNA clone UI-R-FJ0-cpz-e-08-0-UI 3', mRNA sequence

GenBank: CA505718.1

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LOCUS       CA505718                 709 bp    mRNA    linear   EST 27-JAN-2011
DEFINITION  UI-R-FJ0-cpz-e-08-0-UI.s1 UI-R-FJ0 Rattus norvegicus cDNA clone
            UI-R-FJ0-cpz-e-08-0-UI 3', mRNA sequence.
ACCESSION   CA505718
VERSION     CA505718.1
DBLINK      BioSample: SAMN00171765
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            Tissue Procurement: Dr. James Lin, Universtiy of Iowa
             cDNA Library preparation: Dr. M. Bento Soares, University of Iowa
             cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa
             DNA Sequencing by: Dr. M. Bento Soares, University of Iowa
             Clone Distribution: Researchers may obtain clones from Research
            Genetics (www.resgen.com).
            
            Seq primer: M13 FORWARD
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-FJ0-cpz-e-08-0-UI"
                     /tissue_type="embryo"
                     /clone_lib="SAMN00171765 UI-R-FJ0"
                     /dev_stage="embryo"
                     /lab_host="DH10B (Life Technologies) (T1 phage resistant)"
                     /note="Vector: pYX-Asc; Site_1: EcoR I; Site_2: Not I;
                     UI-R-FJ0 is a cDNA library containing the following
                     tissue(s): rat embryo. The library was constructed
                     according to Bonaldo, Lennon and Soares, Genome Research,
                     6:791-806, 1996. First strand cDNA synthesis was primed
                     with an oligo-dT primer containing a Not I site. Double
                     stranded cDNA was ligated to an EcoR I adaptor, digested
                     with Not I, and cloned directionally into pT7T3-Pac
                     vector. The oligonucleotide used to prime the synthesis of
                     first-strand cDNA contains a library tag sequence that is
                     located between the Not I site and the (dT)18 tail. The
                     sequence tag for this library is CATCTCTACT. This library
                     was created for the University of Iowa Program for Rat
                     Gene Discovery and Mapping (Val Sheffield, Bento Soares
                     and Tom Casavant);
                     TAG_LIB=UI-R-FJ0;
                     TAG_SEQ=CATCTCTACT;
                     TAG_TISSUE=rat-embryo"
ORIGIN      
        1 tttttttttt ttttttttgg tggggggagg ggtgttgttc ttttggttta ttttatccta
       61 tctcaaccta ttacatgggt ttgcaaagga agcccctttc tgcctcccta gctataggta
      121 gaaaagtctg gctgatgact attttgtctg agctgtgata ctattttaaa aggatgggcg
      181 ggagaggttc ataaggagag cagggaagtc cagagatagg ttgacaggga aggggactaa
      241 gttgcatgga ggtatgaagt aaacacaggc atagaaatga aaaatctgtc ctcatcagca
      301 tcccttcttg tcccttctaa gcaaggtata aggaataagg gaaatgacat agatacccgg
      361 ttgtggccca aagccttacc cttctcaggg ctcacaacat gatagcaata agcatatgtt
      421 cacaaatagc aggctgcccg ccagtctgac aaccagtagg tttcctaaag tctggggagg
      481 ctaggcttgt agcccatgca ctgagaagat gcctgggtcc gtaggcagtt cttgaggtct
      541 ttaattggag ccaataacat tgacctagcc aaaagtcaat ggactgagtg caaccaggca
      601 cgaccagact tgatgagggt tcactagagg actgtctccg aggcaagagg agacatacca
      661 tgtgaacana acatactcct gtttgccctt gtcctcatcc acacccagt
//
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