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GenBank: CA505956.1
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LOCUS CA505956 686 bp mRNA linear EST 27-JAN-2011 DEFINITION UI-R-FJ0-cqa-c-11-0-UI.s1 UI-R-FJ0 Rattus norvegicus cDNA clone UI-R-FJ0-cqa-c-11-0-UI 3', mRNA sequence. ACCESSION CA505956 VERSION CA505956.1 DBLINK BioSample: SAMN00171765 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 686) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org Tissue Procurement: Dr. James Lin, Universtiy of Iowa cDNA Library preparation: Dr. M. Bento Soares, University of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa DNA Sequencing by: Dr. M. Bento Soares, University of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). The following repetitive elements were found in this cDNA sequence: 1-26, >AT_rich#Low_complexity (matched compliment) Seq primer: M13 FORWARD POLYA=Yes. FEATURES Location/Qualifiers source 1..686 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-FJ0-cqa-c-11-0-UI" /tissue_type="embryo" /clone_lib="SAMN00171765 UI-R-FJ0" /dev_stage="embryo" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /note="Vector: pYX-Asc; Site_1: EcoR I; Site_2: Not I; UI-R-FJ0 is a cDNA library containing the following tissue(s): rat embryo. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CATCTCTACT. This library was created for the University of Iowa Program for Rat Gene Discovery and Mapping (Val Sheffield, Bento Soares and Tom Casavant); TAG_LIB=UI-R-FJ0; TAG_SEQ=CATCTCTACT; TAG_TISSUE=rat-embryo" ORIGIN 1 tttttttttt ttttttttaa aaataacagc aatatttata gactctttga cattaaatct 61 tttataaagc catatgctac acatataaca ccttctctct ctgtaaggtt gacattattc 121 agagagacag ccagaccaca aaccacaaaa caacagtcag acaacaagca ctttacagcg 181 cccaccacag tctgtacact aagaacagtc ctaagacggc atccgccttc tccttcttca 241 atcaggatga agctgtcact gacattccag tatcatgctg acgtggttgt cattgtttca 301 agtcacacca aactgacatc agagagtaca aggttcttta atcaaacaaa aaattagtct 361 tatttaaatt aagagaaatt cacttaaact aaaaataatt tatttgagct gagagtttta 421 aatacctaac acattttttc agggaaatgt tggtaccatc tctgagccag ctttccttcc 481 tccagactgt atctgtataa gaatatataa tttacttatc tctcagtcat ttcagaaacc 541 accgtatctc tgcctatact taggaattcc tacagacgac ctgctcggta acaactctta 601 ctatgataag atgctttctt tgtaccttaa tcctccaaga acacatcana aatggacatt 661 ctatgacaga gtcagtcatt tatctc //
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