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UI-R-FJ0-cqa-c-11-0-UI.s1 UI-R-FJ0 Rattus norvegicus cDNA clone UI-R-FJ0-cqa-c-11-0-UI 3', mRNA sequence

GenBank: CA505956.1

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LOCUS       CA505956                 686 bp    mRNA    linear   EST 27-JAN-2011
DEFINITION  UI-R-FJ0-cqa-c-11-0-UI.s1 UI-R-FJ0 Rattus norvegicus cDNA clone
            UI-R-FJ0-cqa-c-11-0-UI 3', mRNA sequence.
ACCESSION   CA505956
VERSION     CA505956.1
DBLINK      BioSample: SAMN00171765
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            Tissue Procurement: Dr. James Lin, Universtiy of Iowa
             cDNA Library preparation: Dr. M. Bento Soares, University of Iowa
             cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa
             DNA Sequencing by: Dr. M. Bento Soares, University of Iowa
             Clone Distribution: Researchers may obtain clones from Research
            Genetics (www.resgen.com).
             The following repetitive elements were found in this cDNA
            sequence: 1-26, >AT_rich#Low_complexity (matched compliment)
            Seq primer: M13 FORWARD
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-FJ0-cqa-c-11-0-UI"
                     /tissue_type="embryo"
                     /clone_lib="SAMN00171765 UI-R-FJ0"
                     /dev_stage="embryo"
                     /lab_host="DH10B (Life Technologies) (T1 phage resistant)"
                     /note="Vector: pYX-Asc; Site_1: EcoR I; Site_2: Not I;
                     UI-R-FJ0 is a cDNA library containing the following
                     tissue(s): rat embryo. The library was constructed
                     according to Bonaldo, Lennon and Soares, Genome Research,
                     6:791-806, 1996. First strand cDNA synthesis was primed
                     with an oligo-dT primer containing a Not I site. Double
                     stranded cDNA was ligated to an EcoR I adaptor, digested
                     with Not I, and cloned directionally into pT7T3-Pac
                     vector. The oligonucleotide used to prime the synthesis of
                     first-strand cDNA contains a library tag sequence that is
                     located between the Not I site and the (dT)18 tail. The
                     sequence tag for this library is CATCTCTACT. This library
                     was created for the University of Iowa Program for Rat
                     Gene Discovery and Mapping (Val Sheffield, Bento Soares
                     and Tom Casavant);
                     TAG_LIB=UI-R-FJ0;
                     TAG_SEQ=CATCTCTACT;
                     TAG_TISSUE=rat-embryo"
ORIGIN      
        1 tttttttttt ttttttttaa aaataacagc aatatttata gactctttga cattaaatct
       61 tttataaagc catatgctac acatataaca ccttctctct ctgtaaggtt gacattattc
      121 agagagacag ccagaccaca aaccacaaaa caacagtcag acaacaagca ctttacagcg
      181 cccaccacag tctgtacact aagaacagtc ctaagacggc atccgccttc tccttcttca
      241 atcaggatga agctgtcact gacattccag tatcatgctg acgtggttgt cattgtttca
      301 agtcacacca aactgacatc agagagtaca aggttcttta atcaaacaaa aaattagtct
      361 tatttaaatt aagagaaatt cacttaaact aaaaataatt tatttgagct gagagtttta
      421 aatacctaac acattttttc agggaaatgt tggtaccatc tctgagccag ctttccttcc
      481 tccagactgt atctgtataa gaatatataa tttacttatc tctcagtcat ttcagaaacc
      541 accgtatctc tgcctatact taggaattcc tacagacgac ctgctcggta acaactctta
      601 ctatgataag atgctttctt tgtaccttaa tcctccaaga acacatcana aatggacatt
      661 ctatgacaga gtcagtcatt tatctc
//
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