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GenBank: CA507895.1
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LOCUS CA507895 668 bp mRNA linear EST 27-JAN-2011 DEFINITION UI-R-FS1-cql-l-08-0-UI.s1 NCI_CGAP_FS1 Rattus norvegicus cDNA clone IMAGE:7364602 3', mRNA sequence. ACCESSION CA507895 VERSION CA507895.1 DBLINK BioSample: SAMN00171659 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 668) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Daniela S. Gerhard, Ph.D. Office of Cancer Genomics National Cancer Institute / NIH Bldg. 31 Rm10A07 Bethesda, MD 20892 Email: cgapbs-r@mail.nih.gov Oligo-dT track not found, Not I site shown in beginning of sequence is likely internal to the message. cDNA Library Preparation: M.B. Soares Lab Clone distribution: clones will be available through IMAGE (http://image.llnl.gov) Seq primer: M13 FORWARD POLYA=Yes. FEATURES Location/Qualifiers source 1..668 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="IMAGE:7364602" /clone_lib="SAMN00171659 NCI_CGAP_FS1" /dev_stage="ADULT" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; UI-R-FS1 is a normalized cDNA library constructed from Swarm Rat Chondrosarcoma . The library was constructed and normalized according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. The Rat SRC-JWS Cell Line tumor was provided by Dr Jeff Stevens of the University of Iowa. TAG_LIB=UI-R-FS1; TAG_SEQ=AGCCGCCGAT; TAG_TISSUE=Rat SRC-JWS Cell Line" ORIGIN 1 tttttttttt ttttttttaa aataagtgca tacagatacg gctagaagca tttgtttgcc 61 aagtgctctt aacaaaaaaa aaaaaaaaca aaaacaaaaa accaaaaaca gaaaaataac 121 aaaacaaaaa aataaaagca gccaagtagc agcctccaca aaacagcaac tgtcacccga 181 agagtcaagc acagacagcc taacaacgtt aacaaaagaa gaaaatgcct taaagtacat 241 accttttgtc gtcaaaaaaa aaaaatatag aaacacaatg tattcaaaaa aatcaaaatt 301 atacagccat gttaatgaag tctacatttc ccttgtcttg gatatatata tttatatata 361 tatatttatg tatgtatgga gatctatata cagttcaaca agtttgagtt aaaggtaagc 421 gtgggctttt ctgagcacgg aaagtcacga gtctctctcc ttcttcactt tcacatctcc 481 ggcgaggata gtagcaatct ctccttgcat gaaggcccag ggaacgttgg agcccaccag 541 aggacacttc tccccgcttg gacaatagac ctctccgcta gcgccctgct gtttaatgct 601 ttgtctggag caagggaaac agaacttgtg cgaaggtacg gatgggcact gcacaaagtg 661 ggtatcct //
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