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UI-R-FS0-cqq-k-05-0-UI.s1 NCI_CGAP_FS0 Rattus norvegicus cDNA clone IMAGE:7356895 3', mRNA sequence

GenBank: CA509348.1

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LOCUS       CA509348                 738 bp    mRNA    linear   EST 28-JAN-2011
DEFINITION  UI-R-FS0-cqq-k-05-0-UI.s1 NCI_CGAP_FS0 Rattus norvegicus cDNA clone
            IMAGE:7356895 3', mRNA sequence.
ACCESSION   CA509348
VERSION     CA509348.1
DBLINK      BioSample: SAMN00171766
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Daniela S. Gerhard, Ph.D.
            Office of Cancer Genomics
            National Cancer Institute / NIH
            Bldg. 31 Rm10A07 Bethesda, MD 20892
            Email: cgapbs-r@mail.nih.gov
            Oligo-dT track not found, Not I site shown in beginning of sequence
            is likely internal to the message. cDNA Library Preparation: M.B.
            Soares Lab Clone distribution: clones will be available through
            IMAGE (http://image.llnl.gov)
            Seq primer: M13 FORWARD
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="IMAGE:7356895"
                     /clone_lib="SAMN00171766 NCI_CGAP_FS0"
                     /dev_stage="ADULT"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     UI-R-FS0 is a cDNA library constructed from Swarm Rat
                     Chondrosarcoma. The library was constructed according to
                     Bonaldo, Lennon and Soares, Genome Research, 6:791-806,
                     1996. First strand cDNA synthesis was primed with an
                     oligo-dT primer containing a Not I site. Double stranded
                     cDNA was ligated to an EcoR I adaptor, digested with Not
                     I, and cloned directionally into pT7T3-Pac vector. The
                     oligonucleotide used to prime the synthesis of
                     first-strand cDNA contains a library tag sequence that is
                     located between the Not I site and the (dT)18 tail. The
                     sequence tag for this library is AGCCGCCGAT. The tumor was
                     provided by Dr Jeff Stevens from University of Iowa.
                     TAG_LIB=UI-R-FS0;
                     TAG_SEQ=AGCCGCCGAT;
                     TAG_TISSUE=Rat SRC-JWS Cell Line"
ORIGIN      
        1 tttttttttt ttttttttga cttagcanag gctctttatt aggtgggaca gttgaatgaa
       61 gccagtgggg gaggggatct gtgatagccg ctgtaggaac tggcaaggcc tggagtccac
      121 ctgacatggc aggaggcact ggacctgcag gtagcacgca gcctctgggc cggctgggaa
      181 ctgccacagc caggacagat aggggctcca gcatcactag agcagagccg ggagaggccc
      241 tcagaaccca aattctctgc aaacctgggt agacacccat tagacactga gggttaaaga
      301 gatctgactg agaagagagg gacagtccct gtagtgtttc acagcactgc tataaaaccc
      361 aaggatgcca caggctgagg tcctggcact ctcctgaggg gatggacaga ggtgcaggcg
      421 ccagcagcag gtgcccatgt gggcagccaa gggtagtgca atggaaacac acagtggctg
      481 gaggctcccg acgaggagtc aggctgcaga accgttnaca ggcatgagaa gctctgcctg
      541 ggaagtgatc acggaaggct gtccctaagg ttctggggga ggcatgtgca ctctgagagg
      601 ctggagactg ggagctcctg ttgctgtgac cacgaagctg cctctgatgc ctcaggaact
      661 gtgtctgtta cctgngttgg gcagcttggg ggttaaagag gacacagcga ggtctggttt
      721 gttgggtctc tngtctcc
//
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