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GenBank: CA509348.1
FASTA Graphics
LOCUS CA509348 738 bp mRNA linear EST 28-JAN-2011 DEFINITION UI-R-FS0-cqq-k-05-0-UI.s1 NCI_CGAP_FS0 Rattus norvegicus cDNA clone IMAGE:7356895 3', mRNA sequence. ACCESSION CA509348 VERSION CA509348.1 DBLINK BioSample: SAMN00171766 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 738) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Daniela S. Gerhard, Ph.D. Office of Cancer Genomics National Cancer Institute / NIH Bldg. 31 Rm10A07 Bethesda, MD 20892 Email: cgapbs-r@mail.nih.gov Oligo-dT track not found, Not I site shown in beginning of sequence is likely internal to the message. cDNA Library Preparation: M.B. Soares Lab Clone distribution: clones will be available through IMAGE (http://image.llnl.gov) Seq primer: M13 FORWARD POLYA=Yes. FEATURES Location/Qualifiers source 1..738 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="IMAGE:7356895" /clone_lib="SAMN00171766 NCI_CGAP_FS0" /dev_stage="ADULT" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; UI-R-FS0 is a cDNA library constructed from Swarm Rat Chondrosarcoma. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AGCCGCCGAT. The tumor was provided by Dr Jeff Stevens from University of Iowa. TAG_LIB=UI-R-FS0; TAG_SEQ=AGCCGCCGAT; TAG_TISSUE=Rat SRC-JWS Cell Line" ORIGIN 1 tttttttttt ttttttttga cttagcanag gctctttatt aggtgggaca gttgaatgaa 61 gccagtgggg gaggggatct gtgatagccg ctgtaggaac tggcaaggcc tggagtccac 121 ctgacatggc aggaggcact ggacctgcag gtagcacgca gcctctgggc cggctgggaa 181 ctgccacagc caggacagat aggggctcca gcatcactag agcagagccg ggagaggccc 241 tcagaaccca aattctctgc aaacctgggt agacacccat tagacactga gggttaaaga 301 gatctgactg agaagagagg gacagtccct gtagtgtttc acagcactgc tataaaaccc 361 aaggatgcca caggctgagg tcctggcact ctcctgaggg gatggacaga ggtgcaggcg 421 ccagcagcag gtgcccatgt gggcagccaa gggtagtgca atggaaacac acagtggctg 481 gaggctcccg acgaggagtc aggctgcaga accgttnaca ggcatgagaa gctctgcctg 541 ggaagtgatc acggaaggct gtccctaagg ttctggggga ggcatgtgca ctctgagagg 601 ctggagactg ggagctcctg ttgctgtgac cacgaagctg cctctgatgc ctcaggaact 661 gtgtctgtta cctgngttgg gcagcttggg ggttaaagag gacacagcga ggtctggttt 721 gttgggtctc tngtctcc //
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