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GenBank: CA539533.1
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LOCUS CA539533 634 bp mRNA linear EST 16-DEC-2010 DEFINITION C0280B02-5N NIA Mouse 7.5-dpc Whole Embryo cDNA Library (Long) Mus musculus cDNA clone NIA:C0280B02 IMAGE:30018253 5', mRNA sequence. ACCESSION CA539533 VERSION CA539533.1 DBLINK BioSample: SAMN00169965 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 634) AUTHORS Piao,Y., Kargul,G.J., Dudekula,D.B., Qian,Y., Tanaka,T., Lim,M.K., Luo,A. and Ko,M.S.H. TITLE Systematic Analyses of NIA Mouse 7.5-dpc Whole Embryo cDNA Library (Long) JOURNAL Unpublished COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: C0280 row: B column: 02 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..634 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:C0280B02-5N" /db_xref="taxon:10090" /clone="NIA:C0280B02 IMAGE:30018253" /tissue_type="whole embryo including extraembryonic tissues at 7.5-days postcoitum" /clone_lib="SAMN00169965 NIA Mouse 7.5-dpc Whole Embryo cDNA Library (Long)" /dev_stage="7.5-days postcoitum" /lab_host="DH10B" /note="Vector: pSPORT1 (Invitrogen); Site_1: SalI; Site_2: NotI; Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were extracted from a pool of four embryos at 7.5-days postcoitum. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 7 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.2 kb. The library was constructed by Yulan Piao (NIA)." ORIGIN 1 taaaaatcgc agaaaacgtc ttacggtgcc acagcaaaca acattgaact cactcccctg 61 tgctcgcgtg tggaatttct cttgtgtgga atttctgagt cagaatagtt tctccagtca 121 ctagcacata cagccatatt ttgcattttt aacagcacca ctgtaccagt gtgtggatga 181 gtcttgtttt agaaaccatc tgagtgagta agttgggcta gcaagtcttc ctttcttaca 241 atttttttta tttagtcatt tgtggattga caactttcag accacatgtt cttgcatgtc 301 tgaaaataag tagataatgg cagtttagca gaggctttga gttaagctct gacttcagct 361 cctgcctcgg tagcagacag tcaagttact atctacgctt aattcacaca tgaggttgtt 421 tgtaagaaca tttctgcaaa ccaaagcaat agctaaagta aagaatgaaa acaatttgtt 481 tgtttgcttg cttgtttgtt tttgagacaa ggtttctctg tgccctggct gtcctcaaat 541 gaacttactc tgttagccag gctggccttg aactcccgta gatccacctg tctgtgcctc 601 tcgagtactg gcattaaagg catgcaccac caca //
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