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C0280B02-5N NIA Mouse 7.5-dpc Whole Embryo cDNA Library (Long) Mus musculus cDNA clone NIA:C0280B02 IMAGE:30018253 5', mRNA sequence

GenBank: CA539533.1

FASTA Graphics 

LOCUS       CA539533                 634 bp    mRNA    linear   EST 16-DEC-2010
DEFINITION  C0280B02-5N NIA Mouse 7.5-dpc Whole Embryo cDNA Library (Long) Mus
            musculus cDNA clone NIA:C0280B02 IMAGE:30018253 5', mRNA sequence.
ACCESSION   CA539533
VERSION     CA539533.1
DBLINK      BioSample: SAMN00169965
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Piao,Y., Kargul,G.J., Dudekula,D.B., Qian,Y., Tanaka,T., Lim,M.K.,
            Luo,A. and Ko,M.S.H.
  TITLE     Systematic Analyses of NIA Mouse 7.5-dpc Whole Embryo cDNA Library
            (Long)
  JOURNAL   Unpublished
COMMENT     Contact: Dawood B. Dudekula
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            Plate: C0280  row: B  column: 02
            Seq primer: M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:C0280B02-5N"
                     /db_xref="taxon:10090"
                     /clone="NIA:C0280B02 IMAGE:30018253"
                     /tissue_type="whole embryo including extraembryonic
                     tissues at 7.5-days postcoitum"
                     /clone_lib="SAMN00169965 NIA Mouse 7.5-dpc Whole Embryo
                     cDNA Library (Long)"
                     /dev_stage="7.5-days postcoitum"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1 (Invitrogen); Site_1: SalI; Site_2:
                     NotI; Mouse cDNA project by the Laboratory of Genetics,
                     National Institute on Aging (NIA), Intramural Research
                     Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is
                     a long-transcript enriched cDNA library (Ref. Genome Res.
                     11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were
                     extracted from a pool of four embryos at 7.5-days
                     postcoitum. Double-stranded cDNAs were synthesized with an
                     Oligo(dT) primer [Invitrogen:
                     5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from
                     7 ug of total RNA, treated with T4 DNA polymerase, and
                     purified by ethanol-precipitation. The cDNAs were ligated
                     to Lone-linker LL-Sal4, purified by phenol/chloroform, and
                     separated from free linkers by Centricon 100. Then, the
                     cDNAs were amplified by long-range high fidelity PCR using
                     Ex Taq polymerase (Takara) with a primer Sal4-S. The
                     products were purified by phenol/chloroform and Centricon
                     100. The cDNAs were digested with SalI and NotI enzymes
                     and cloned into SalI/NotI site of pSPORT1 plasmid vector.
                     The DH10B E. coli host was transformed with the ligation
                     mixture by the standard chemical method. The average
                     insert size is about 2.2 kb. The library was constructed
                     by Yulan Piao (NIA)."
ORIGIN      
        1 taaaaatcgc agaaaacgtc ttacggtgcc acagcaaaca acattgaact cactcccctg
       61 tgctcgcgtg tggaatttct cttgtgtgga atttctgagt cagaatagtt tctccagtca
      121 ctagcacata cagccatatt ttgcattttt aacagcacca ctgtaccagt gtgtggatga
      181 gtcttgtttt agaaaccatc tgagtgagta agttgggcta gcaagtcttc ctttcttaca
      241 atttttttta tttagtcatt tgtggattga caactttcag accacatgtt cttgcatgtc
      301 tgaaaataag tagataatgg cagtttagca gaggctttga gttaagctct gacttcagct
      361 cctgcctcgg tagcagacag tcaagttact atctacgctt aattcacaca tgaggttgtt
      421 tgtaagaaca tttctgcaaa ccaaagcaat agctaaagta aagaatgaaa acaatttgtt
      481 tgtttgcttg cttgtttgtt tttgagacaa ggtttctctg tgccctggct gtcctcaaat
      541 gaacttactc tgttagccag gctggccttg aactcccgta gatccacctg tctgtgcctc
      601 tcgagtactg gcattaaagg catgcaccac caca
//
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