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AGENCOURT_18672698 NIH_MGC_189 Mus musculus cDNA clone IMAGE:30840140 5', mRNA sequence

GenBank: CK790536.1

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LOCUS       CK790536                 797 bp    mRNA    linear   EST 25-FEB-2004
DEFINITION  AGENCOURT_18672698 NIH_MGC_189 Mus musculus cDNA clone
            IMAGE:30840140 5', mRNA sequence.
ACCESSION   CK790536
VERSION     CK790536.1
DBLINK      BioSample: SAMN00174735
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 797)
  CONSRTM   National Institutes of Health, Mammalian Gene Collection (MGC)
  TITLE     NIH-MGC EST Sequencing Project
  JOURNAL   Unpublished
  REMARK    http://mgc.nci.nih.gov/
COMMENT     Contact: Daniela S. Gerhard, Ph.D.
            Office of Cancer Genomics
            National Cancer Institute / NIH
            Bldg. 31 Rm10A07 Bethesda, MD 20892
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: Shioko Kimura/Atsushi Yamada, (NCI,CCR)
             cDNA Library Preparation: Express Genomics
             cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
             DNA Sequencing by: Agencourt Bioscience Corporation
             Clone distribution: MGC clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            http://image.llnl.gov
            
            Plate: NDAM1133  row: b  column: 21
            High quality sequence stop: 608.
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:30840140"
                     /tissue_type="Pooled thyroids from 5 mice"
                     /clone_lib="SAMN00174735 NIH_MGC_189"
                     /lab_host="DH10B TonA"
                     /note="Organ: thyroid; Vector: pExpress-1; Site_1: NotI;
                     Site_2: NotI; RNA obtained from 5 normal wild-type mice.
                     cDNA was primed using oligo-dT primer:
                     5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into
                     the EcoRV/NotI sites of pExpress-1. Size-selection 1.4 kb
                     resulted in an average insert size of 1.2 kb. This
                     primary, nanoquantity library is normalized to Cot5
                     (non-normalized primary library is NIH_MGC_230) and was
                     constructed by Express Genomics (Frederick, MD). Note:
                     this is a NIH_MGC library"
ORIGIN      
        1 ggattcccgg gattacagag ttaatgactt gatccccttc aagggtgcat aaaatagtca
       61 ggaattcagc tagctgttta ataaataatg cattaggaac atttctaaag cccggtgaag
      121 tggtgcatac ctgtcatccc agcaattcag aagctgaggc aggaggatta ctatgagtgt
      181 acagccagcc tgggccatgt aacaagactc tgcttcaaac aaaacctatt ttaagtatgt
      241 aatgagtaat tcctatgtta cactttaaag acaaaagtgt tgtgagcatg cttcggaagt
      301 cattttctaa acatgtctag aaagccatta cgcagactaa agtagagatg catcatcctt
      361 tcaaagcaca gactgggaag gcctttgtgt tataaccatc ataagtcaac agcctaagct
      421 atgactcaat acctcgaaac aattgctgct acttcatgca aggattttaa atgtcgagtt
      481 aggtgatttc ctgatagttc aatcatccct gagccttaaa atgttctctc tcttgaaagc
      541 ttcatcagaa tgaaattctc tctagtttgc ttgggtgtac acatatctgg tccacaggga
      601 acctgaagat ggtcacacac atacacctcc cataagatgt aatattcctt cactttctac
      661 tatgcgcttt tttttctact agctgaaaag ttccttttat cangtgtaac tactgatgct
      721 gtggtgtatt ggacactagc antaaaacgt gaagagaaaa aaaaaaaaaa aaaaaaaagg
      781 cgcgctatac tcagatg
//
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