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GenBank: F27815.1
FASTA Graphics
LOCUS F27815 306 bp mRNA linear EST 29-JAN-2011 DEFINITION HSPD16057 HM3 Homo sapiens cDNA clone s4000021H09, mRNA sequence. ACCESSION F27815 VERSION F27815.1 DBLINK BioSample: SAMN00155451 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 306) AUTHORS Lanfranchi,G., Muraro,T., Caldara,F., Pacchioni,B., Pallavicini,A., Pandolfo,D., Toppo,S., Trevisan,S., Scarso,S. and Valle,G. TITLE Identification of 4370 expressed sequence tags from a 3'-end-specific cDNA library of human skeletal muscle by DNA sequencing and filter hybridization JOURNAL Genome Res. 6 (1), 35-42 (1996) PUBMED 8681137 COMMENT Contact: Valle G. CRIBI Biotechnology Centre University of Padua Via Trieste 75, 35121 Padua, Italy ABI Chromatograms and other information are available on WWW at http://grup.bio.unipd.it. FEATURES Location/Qualifiers source 1..306 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="s4000021H09" /sex="female" /tissue_type="pectoral muscle (after mastectomy)" /clone_lib="SAMN00155451 HM3" /note="Vector: pcDNAII (Invitrogen); Site_1: BstXI; Site_2: NotI; The library was constructed by G. Lanfranchi. This library is not subtracted nor normalized. The first strand cDNA was primed with a biotinylated oligo-dT-NotI primer (5'-biotin-AACCCGGCTCGAGCGGCCGCTTTTTTTTTTTTTTTTTT-3'). The ds cDNA was sonicated and size-selected in the range 350-550 bp. The 3' specific fragments were selected by streptavidin coated magnetic beads, ligated to non-palindromic BstXI adapters, NotI digested and directionally cloned into BstXI-NotI cut pcDNAII vector." ORIGIN 1 cttcttcttt ccaggacctg acagaagtca gggaagagtc cctgggttat gtccaaactt 61 agcacctgca attgttggga tgtggatgga tgtgtgcata agagagagag agaatatgtg 121 tgtgtgtgtg tgcgtctgcg agcgcacaca catgcacaag tgcgaaggag ttgcggttgc 181 tccatgttct gacttagggc aatttgattc tgcacttggg gtctgtctgt acagttactc 241 atgtcattgt aatgatttca ctcctaactg tgacattttt atcaaatgtg tgaataaata 301 cataaa //
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