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HSPD16057 HM3 Homo sapiens cDNA clone s4000021H09, mRNA sequence

GenBank: F27815.1

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LOCUS       F27815                   306 bp    mRNA    linear   EST 29-JAN-2011
DEFINITION  HSPD16057 HM3 Homo sapiens cDNA clone s4000021H09, mRNA sequence.
ACCESSION   F27815
VERSION     F27815.1
DBLINK      BioSample: SAMN00155451
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Lanfranchi,G., Muraro,T., Caldara,F., Pacchioni,B., Pallavicini,A.,
            Pandolfo,D., Toppo,S., Trevisan,S., Scarso,S. and Valle,G.
  TITLE     Identification of 4370 expressed sequence tags from a
            3'-end-specific cDNA library of human skeletal muscle by DNA
            sequencing and filter hybridization
  JOURNAL   Genome Res. 6 (1), 35-42 (1996)
   PUBMED   8681137
COMMENT     Contact: Valle G.
            CRIBI Biotechnology Centre
            University of Padua
            Via Trieste 75, 35121 Padua, Italy
            ABI Chromatograms and other information are available on WWW at
            http://grup.bio.unipd.it.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="s4000021H09"
                     /sex="female"
                     /tissue_type="pectoral muscle (after mastectomy)"
                     /clone_lib="SAMN00155451 HM3"
                     /note="Vector: pcDNAII (Invitrogen); Site_1: BstXI;
                     Site_2: NotI; The library was constructed by G.
                     Lanfranchi. This library is not subtracted nor normalized.
                     The first strand cDNA was primed with a biotinylated
                     oligo-dT-NotI primer
                     (5'-biotin-AACCCGGCTCGAGCGGCCGCTTTTTTTTTTTTTTTTTT-3'). The
                     ds cDNA was sonicated and size-selected in the range
                     350-550 bp. The 3' specific fragments were selected by
                     streptavidin coated magnetic beads, ligated to
                     non-palindromic BstXI adapters, NotI digested and
                     directionally cloned into BstXI-NotI cut pcDNAII vector."
ORIGIN      
        1 cttcttcttt ccaggacctg acagaagtca gggaagagtc cctgggttat gtccaaactt
       61 agcacctgca attgttggga tgtggatgga tgtgtgcata agagagagag agaatatgtg
      121 tgtgtgtgtg tgcgtctgcg agcgcacaca catgcacaag tgcgaaggag ttgcggttgc
      181 tccatgttct gacttagggc aatttgattc tgcacttggg gtctgtctgt acagttactc
      241 atgtcattgt aatgatttca ctcctaactg tgacattttt atcaaatgtg tgaataaata
      301 cataaa
//
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