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yp44f05.s1 Soares retina N2b5HR Homo sapiens cDNA clone IMAGE:190305 3', mRNA sequence

GenBank: H29886.1

FASTA Graphics 

LOCUS       H29886                   435 bp    mRNA    linear   EST 16-AUG-1995
DEFINITION  yp44f05.s1 Soares retina N2b5HR Homo sapiens cDNA clone
            IMAGE:190305 3', mRNA sequence.
ACCESSION   H29886
VERSION     H29886.1
DBLINK      BioSample: SAMN00154917
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            Insert Size: 895
            High quality sequence stops: 248
            Source: IMAGE Consortium, LLNL
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 895   Std Error: 0.00
            Seq primer: Promega -21m13
            High quality sequence stop: 248.
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:3850554"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:190305"
                     /sex="male"
                     /tissue_type="retina"
                     /clone_lib="SAMN00154917 Soares retina N2b5HR"
                     /dev_stage="55 year old"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: eye; Vector: pT7T3D (Pharmacia) with a
                     modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
                     strand cDNA was primed with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). The retinas were obtained from a 55 year old
                     Caucasian and total cellular poly(A)+ RNA was extracted 6
                     hrs after their removal. The retina RNA was kindly
                     provided by Roderick R. McInnes M.D. Ph.D. from the
                     University of Toronto. Library constructed by Bento Soares
                     and M.Fatima Bonaldo."
ORIGIN      
        1 cctattaatn tatgnnattt attttcctta aagcagacat aaaattacat ttggaagaaa
       61 ataactttga tgtattattt aaaccttcta ggataaaaat acatgcaagt tcttctaatc
      121 attcagaact aagaagacat atgaggtgta aaattgatat ttatttttgt tattctcaat
      181 ggaagatcag agttatagta tgcttctcag aaactattgt aacagtttac ttcatgggta
      241 caaaattgcc ataggaaatt tttcccgggt gaatgggcag acgggggcag ctaatatttc
      301 catggcttat ttcctcaaat gtaggatttc caaagggggg gnttttgncc gggaaaaata
      361 ccctaacncc cggcaatggg gggatacggt tgggaatgta gggncctttn atttttcccc
      421 ggntggttaa tgggt
//
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