LOCUS H49454 483 bp mRNA linear EST 18-SEP-1995
DEFINITION yo23g10.r1 Soares adult brain N2b5HB55Y Homo sapiens cDNA clone
IMAGE:178818 5', mRNA sequence.
ACCESSION H49454
VERSION H49454.1
DBLINK BioSample: SAMN00154914
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 483)
AUTHORS Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
Wilson,R.
TITLE The WashU-Merck EST Project
JOURNAL Unpublished
COMMENT Contact: Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: est@watson.wustl.edu
Insert Size: 1005
High quality sequence stops: 316
Source: IMAGE Consortium, LLNL
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Insert Length: 1005 Std Error: 0.00
Seq primer: M13RP1
High quality sequence stop: 316.
FEATURES Location/Qualifiers
source 1..483
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="GDB:3841014"
/db_xref="taxon:9606"
/clone="IMAGE:178818"
/sex="male"
/clone_lib="SAMN00154914 Soares adult brain N2b5HB55Y"
/dev_stage="55-year old"
/lab_host="DH10B (ampicillin resistant)"
/note="Organ: brain; Vector: pT7T3D (Pharmacia) with a
modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
strand cDNA was primed with a Not I - oligo(dT) primer [5'
TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was size selected, ligated to Eco RI
adapters (Pharmacia), digested with Not I and cloned into
the Not I and Eco RI sites of a modified pT7T3 vector
(Pharmacia). Library went through one round of
normalization to a Cot = 53. Library constructed by Bento
Soares and M.Fatima Bonaldo. The adult brain RNA was
provided by Dr. Donald H. Gilden. Tissue was acquired
17-18 hours after death which occurred in consequence of a
ruptured aortic aneurysm. RNA was prepared from a pool of
tissues representing the following areas of the brain:
frontal, parietal, temporal and occipital cortex from the
left and right hemispheres, subcortical white matter,
basal ganglia, thalamus, cerebellum, midbrain, pons and
medulla."
ORIGIN
1 tggaagggtg aatagtttcc taatggttaa aaaccaactg tgaaaggaac cacctgtgtg
61 gttgggttca ctcattctca gattaaattg ccacttaaag aaataacgtg catgctttaa
121 aaaacacagt cacgcaccaa gcaggcaaat agctttagtc cttctcacct cacatcacag
181 ttgttctgca aagtaaaatt ttttggttaa gagcgtgtcc agtagtaatg tgcttgttag
241 ctgtttctca agaccaacag aagatttttt cagttacttt ccccccatgt attttgtatg
301 catatgattg tccgtgataa ttgggctact tttccattgt ttcctcctta aatcgtttag
361 gcngggctgn agggccacat tccctgggac gggnggaccc cttcctcttc agaggtccct
421 gggtacacag ctccgaggtt gatctttttc tgccntgagg ttaaaggttc tatggccctt
481 ttt
//