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yo23g10.r1 Soares adult brain N2b5HB55Y Homo sapiens cDNA clone IMAGE:178818 5', mRNA sequence

GenBank: H49454.1

FASTA Graphics 

LOCUS       H49454                   483 bp    mRNA    linear   EST 18-SEP-1995
DEFINITION  yo23g10.r1 Soares adult brain N2b5HB55Y Homo sapiens cDNA clone
            IMAGE:178818 5', mRNA sequence.
ACCESSION   H49454
VERSION     H49454.1
DBLINK      BioSample: SAMN00154914
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            Insert Size: 1005
            High quality sequence stops: 316
            Source: IMAGE Consortium, LLNL
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 1005   Std Error: 0.00
            Seq primer: M13RP1
            High quality sequence stop: 316.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:3841014"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:178818"
                     /sex="male"
                     /clone_lib="SAMN00154914 Soares adult brain N2b5HB55Y"
                     /dev_stage="55-year old"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: brain; Vector: pT7T3D (Pharmacia) with a
                     modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
                     strand cDNA was primed with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library went through one round of
                     normalization to a Cot = 53. Library constructed by Bento
                     Soares and M.Fatima Bonaldo. The adult brain RNA was
                     provided by Dr. Donald H. Gilden. Tissue was acquired
                     17-18 hours after death which occurred in consequence of a
                     ruptured aortic aneurysm. RNA was prepared from a pool of
                     tissues representing the following areas of the brain:
                     frontal, parietal, temporal and occipital cortex from the
                     left and right hemispheres, subcortical white matter,
                     basal ganglia, thalamus, cerebellum, midbrain, pons and
                     medulla."
ORIGIN      
        1 tggaagggtg aatagtttcc taatggttaa aaaccaactg tgaaaggaac cacctgtgtg
       61 gttgggttca ctcattctca gattaaattg ccacttaaag aaataacgtg catgctttaa
      121 aaaacacagt cacgcaccaa gcaggcaaat agctttagtc cttctcacct cacatcacag
      181 ttgttctgca aagtaaaatt ttttggttaa gagcgtgtcc agtagtaatg tgcttgttag
      241 ctgtttctca agaccaacag aagatttttt cagttacttt ccccccatgt attttgtatg
      301 catatgattg tccgtgataa ttgggctact tttccattgt ttcctcctta aatcgtttag
      361 gcngggctgn agggccacat tccctgggac gggnggaccc cttcctcttc agaggtccct
      421 gggtacacag ctccgaggtt gatctttttc tgccntgagg ttaaaggttc tatggccctt
      481 ttt
//
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