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yr56g07.s1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE:209340 3', mRNA sequence

GenBank: H65511.1

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LOCUS       H65511                   317 bp    mRNA    linear   EST 18-OCT-1995
DEFINITION  yr56g07.s1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone
            IMAGE:209340 3', mRNA sequence.
ACCESSION   H65511
VERSION     H65511.1
DBLINK      BioSample: SAMN00154876
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            Insert Size: 612
            High quality sequence stops: 279
            Source: IMAGE Consortium, LLNL
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 612   Std Error: 0.00
            Seq primer: Promega -21m13
            High quality sequence stop: 279.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:3782181"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:209340"
                     /sex="male"
                     /clone_lib="SAMN00154876 Soares fetal liver spleen 1NFLS"
                     /dev_stage="20 week-post conception fetus"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
                     with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
                     1st strand cDNA was primed with a Pac I - oligo(dT) primer
                     [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Pac I and cloned into the Pac I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization. Library
                     constructed by Bento Soares and M.Fatima Bonaldo."
ORIGIN      
        1 ttaataggaa agtaaagagc actttcccca aacagcaaga acaaaatcgc tttctcttaa
       61 cacattgatt tatttcattt gctaagcaca ggaaccatga aatgcttcat cccattagca
      121 cattaaatat gtgcagaagt aaaattacag ctgaacagca ccttgtactt tgtactcatg
      181 gtgtgtacat ttcagtaggc taggaggaaa aaacgaactg tagaaagata attttatggg
      241 aattaaatta cctgttttgt attgctctga agctgtgcat gtaggctgtg ggatttcaga
      301 gggacttgaa ctgttgc
//
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