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yu21b07.r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE:234421 5', mRNA sequence

GenBank: H94805.1

FASTA Graphics 

LOCUS       H94805                   512 bp    mRNA    linear   EST 25-NOV-1996
DEFINITION  yu21b07.r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone
            IMAGE:234421 5', mRNA sequence.
ACCESSION   H94805
VERSION     H94805.1
DBLINK      BioSample: SAMN00154876
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            High quality sequence stops: 338
            Source: IMAGE Consortium, LLNL
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Putative full length read
            Insert Length: 334   Std Error: 0.00
            Seq primer: M13RP1.
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:3787598"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:234421"
                     /sex="male"
                     /clone_lib="SAMN00154876 Soares fetal liver spleen 1NFLS"
                     /dev_stage="20 week-post conception fetus"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
                     with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
                     1st strand cDNA was primed with a Pac I - oligo(dT) primer
                     [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Pac I and cloned into the Pac I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization. Library
                     constructed by Bento Soares and M.Fatima Bonaldo."
ORIGIN      
        1 taggctacgt ggaagccgat aaggagaatg gcgagaatgt gtgggtggcg ccctccgctc
       61 cgggcccagg tcctgcaccc tgcacaggtg gccccaagcc gctgtcacct gcgtctagcc
      121 aggacaagct gcccccactg cccccactac cgaaccagga agagaactac gtgaccccca
      181 ttggagatgg cccagctgtt gactatgaga accaagatgt ggcttcctct agttggccag
      241 tcatcctgaa gccaaagaag ttgccaaagc ctcctgccaa gcttccaaag ccacccgttg
      301 gacccaagcc agagcccaaa gtctttaatg gtggcttggg cagggaagct gccagttcag
      361 tttcagccca gcctcttctt tccccacagn ccgggctggg cagacatgga cggcagagtt
      421 acagaagaag ctgggagaag aggcggngca ctggtagcat ggtttcggac acaccaggga
      481 ccagcgggtt agttccaggg cnggccaggt gg
//
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