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GenBank: N28028.1
FASTA Graphics
LOCUS N28028 447 bp mRNA linear EST 30-DEC-1995 DEFINITION yx50h10.r1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE:265219 5', mRNA sequence. ACCESSION N28028 VERSION N28028.1 DBLINK BioSample: SAMN00154979 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 447) AUTHORS Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M., Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M., Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F., Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and Wilson,R. TITLE The WashU-Merck EST Project JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu High quality sequence stops: 360 Source: IMAGE Consortium, LLNL This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Insert Length: 1066 Std Error: 0.00 Seq primer: T7 High quality sequence stop: 360. FEATURES Location/Qualifiers source 1..447 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="GDB:3874861" /db_xref="taxon:9606" /clone="IMAGE:265219" /sex="male" /tissue_type="melanocyte" /clone_lib="SAMN00154979 Soares melanocyte 2NbHM" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAGTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library constructed by Bento Soares and M.Fatima Bonaldo. RNA from normal foreskin melanocytes (FS374) was kindly provided by Dr. Anthony P. Albino." ORIGIN 1 ttctactaca gaacatactg tagacagtta ttcagtggtt aacttgtgga aataatcaga 61 agatgctttt cacttttatg actggatata ttttaatatt tatcatatta agagaaactt 121 aggttactta cagctcataa acgttgctat gaacatcatt catttattca cttatttatt 181 tttatggtgt tcctagaaag aatttcctgg actaaaggat ttatgcttta aaaatattgg 241 tagttactgc caagaattgc catccagaaa cagtgtagcc attcataaac tcaacaacaa 301 ggtatgagag tgcctgtttc ctcatatcct ttgtgacacc ggattttatc aagcttaaag 361 ttttttccat tctagggtgg gtgaaaatgg tattccgtta ttggataatg tgtggaaatc 421 aaggtcctaa taaagtttgg ggggagn //
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