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yx50h10.r1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE:265219 5', mRNA sequence

GenBank: N28028.1

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LOCUS       N28028                   447 bp    mRNA    linear   EST 30-DEC-1995
DEFINITION  yx50h10.r1 Soares melanocyte 2NbHM Homo sapiens cDNA clone
            IMAGE:265219 5', mRNA sequence.
ACCESSION   N28028
VERSION     N28028.1
DBLINK      BioSample: SAMN00154979
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            High quality sequence stops: 360
            Source: IMAGE Consortium, LLNL
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 1066   Std Error: 0.00
            Seq primer: T7
            High quality sequence stop: 360.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:3874861"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:265219"
                     /sex="male"
                     /tissue_type="melanocyte"
                     /clone_lib="SAMN00154979 Soares melanocyte 2NbHM"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Vector: pT7T3D (Pharmacia) with a modified
                     polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
                     was primed with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCAGTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library constructed by Bento Soares and
                     M.Fatima Bonaldo. RNA from normal foreskin melanocytes
                     (FS374) was kindly provided by Dr. Anthony P. Albino."
ORIGIN      
        1 ttctactaca gaacatactg tagacagtta ttcagtggtt aacttgtgga aataatcaga
       61 agatgctttt cacttttatg actggatata ttttaatatt tatcatatta agagaaactt
      121 aggttactta cagctcataa acgttgctat gaacatcatt catttattca cttatttatt
      181 tttatggtgt tcctagaaag aatttcctgg actaaaggat ttatgcttta aaaatattgg
      241 tagttactgc caagaattgc catccagaaa cagtgtagcc attcataaac tcaacaacaa
      301 ggtatgagag tgcctgtttc ctcatatcct ttgtgacacc ggattttatc aagcttaaag
      361 ttttttccat tctagggtgg gtgaaaatgg tattccgtta ttggataatg tgtggaaatc
      421 aaggtcctaa taaagtttgg ggggagn
//
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