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yy22g09.s1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE:272032 3', mRNA sequence

GenBank: N31945.1

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LOCUS       N31945                   451 bp    mRNA    linear   EST 10-JAN-1996
DEFINITION  yy22g09.s1 Soares melanocyte 2NbHM Homo sapiens cDNA clone
            IMAGE:272032 3', mRNA sequence.
ACCESSION   N31945
VERSION     N31945.1
DBLINK      BioSample: SAMN00154979
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            High quality sequence stops: 241
            Source: IMAGE Consortium, LLNL
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: m13 -40 forward
            High quality sequence stop: 241.
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:3881674"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:272032"
                     /sex="male"
                     /tissue_type="melanocyte"
                     /clone_lib="SAMN00154979 Soares melanocyte 2NbHM"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Vector: pT7T3D (Pharmacia) with a modified
                     polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
                     was primed with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCAGTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library constructed by Bento Soares and
                     M.Fatima Bonaldo. RNA from normal foreskin melanocytes
                     (FS374) was kindly provided by Dr. Anthony P. Albino."
ORIGIN      
        1 tttttttttt tcactttttt gttgttttna atatattttn ccttcctggt tgctaattta
       61 gactgaattc ttaaggattt atcttgatga cttagaaaaa tacacctgtc ctttctcact
      121 ttgtttcagg tagtacagtc atgaaattga gtaagtcnct taaaaaaatg aaagtccaca
      181 gatcccattg nggaaggttt caggaacccc tccccaggta atgatcacgg gatcttcaac
      241 agctcaagga gtgctccaac agctccaaaa taaccctcgt gttccgaaaa aagngctttc
      301 aactggcccc ttggaccaat aatccaaagc atatgccaaa agccgcatgg natccgtatt
      361 aattctcaag aaatttccaa caaataccac ctgggttaat ggttttcatt aagggcacac
      421 attcttggta ttgngccaat gttgttggtg a
//
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