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yy10a10.r1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE:270810 5', mRNA sequence

GenBank: N42497.1

FASTA Graphics 

LOCUS       N42497                   436 bp    mRNA    linear   EST 25-JAN-1996
DEFINITION  yy10a10.r1 Soares melanocyte 2NbHM Homo sapiens cDNA clone
            IMAGE:270810 5', mRNA sequence.
ACCESSION   N42497
VERSION     N42497.1
DBLINK      BioSample: SAMN00154979
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            Source: IMAGE Consortium, LLNL
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: T7.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:3880452"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:270810"
                     /sex="male"
                     /tissue_type="melanocyte"
                     /clone_lib="SAMN00154979 Soares melanocyte 2NbHM"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Vector: pT7T3D (Pharmacia) with a modified
                     polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
                     was primed with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCAGTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library constructed by Bento Soares and
                     M.Fatima Bonaldo. RNA from normal foreskin melanocytes
                     (FS374) was kindly provided by Dr. Anthony P. Albino."
ORIGIN      
        1 gagagaaaac attagaattt gttccaataa atctacatct gcaaagaatg caggtacaca
       61 gccctcactt gaaagatgct ctctacgatg tcatcactgt gggagcccca gctgcccatt
      121 ttcagggatt taagaatggt ggtcttcgga agctactcca tagatttgaa acagaaagaa
      181 gaaataccgg ataccagttt atttactatt cacctgaaaa cacagccaaa gcaaaggaag
      241 ttctcagcaa catcaatcaa ctacaacctc ttatagcaac ccatgcagac ctactgctta
      301 attctgcaag ccagcattct ccagacagct tgaagaattc tttaaagatg ctttcagaaa
      361 aaacagagct ttttgtacat gccttcaagg atcaacttgt caggagtgct cttttagcac
      421 tctacactgc aaggcc
//
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