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GenBank: N42497.1
FASTA Graphics
LOCUS N42497 436 bp mRNA linear EST 25-JAN-1996 DEFINITION yy10a10.r1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE:270810 5', mRNA sequence. ACCESSION N42497 VERSION N42497.1 DBLINK BioSample: SAMN00154979 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 436) AUTHORS Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M., Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M., Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F., Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and Wilson,R. TITLE The WashU-Merck EST Project JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu Source: IMAGE Consortium, LLNL This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: T7. FEATURES Location/Qualifiers source 1..436 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="GDB:3880452" /db_xref="taxon:9606" /clone="IMAGE:270810" /sex="male" /tissue_type="melanocyte" /clone_lib="SAMN00154979 Soares melanocyte 2NbHM" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAGTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library constructed by Bento Soares and M.Fatima Bonaldo. RNA from normal foreskin melanocytes (FS374) was kindly provided by Dr. Anthony P. Albino." ORIGIN 1 gagagaaaac attagaattt gttccaataa atctacatct gcaaagaatg caggtacaca 61 gccctcactt gaaagatgct ctctacgatg tcatcactgt gggagcccca gctgcccatt 121 ttcagggatt taagaatggt ggtcttcgga agctactcca tagatttgaa acagaaagaa 181 gaaataccgg ataccagttt atttactatt cacctgaaaa cacagccaaa gcaaaggaag 241 ttctcagcaa catcaatcaa ctacaacctc ttatagcaac ccatgcagac ctactgctta 301 attctgcaag ccagcattct ccagacagct tgaagaattc tttaaagatg ctttcagaaa 361 aaacagagct ttttgtacat gccttcaagg atcaacttgt caggagtgct cttttagcac 421 tctacactgc aaggcc //
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