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yz18g09.s1 Soares_multiple_sclerosis_2NbHMSP Homo sapiens cDNA clone IMAGE:283456 3', mRNA sequence

GenBank: N50641.1

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LOCUS       N50641                   490 bp    mRNA    linear   EST 14-FEB-1996
DEFINITION  yz18g09.s1 Soares_multiple_sclerosis_2NbHMSP Homo sapiens cDNA
            clone IMAGE:283456 3', mRNA sequence.
ACCESSION   N50641
VERSION     N50641.1
DBLINK      BioSample: SAMN00155002
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: m13 -40 forward
            High quality sequence stop: 257.
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:3901984"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:283456"
                     /sex="male"
                     /tissue_type="multiple sclerosis lesions"
                     /clone_lib="SAMN00155002
                     Soares_multiple_sclerosis_2NbHMSP"
                     /dev_stage="Age 46"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Vector: pT7T3D (Pharmacia) with a modified
                     polylinker V_TYPE: phagemid; Site_1: Not I; Site_2: Eco
                     RI; 1st strand cDNA was primed with a Not I - oligo(dT)
                     primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library went through one round of
                     normalization to a Cot = 5. Library constructed by Bento
                     Soares and M.Fatima Bonaldo. RNA from 4 multiple sclerosis
                     lesions from one patient was kindly provided by Dr. Kevin
                     G. Becker (NINDS/NIH)."
ORIGIN      
        1 nttttttttt acttttaaaa ttttatgttt aacaatttat tctattttaa aatatgtact
       61 gtattttgaa cataactgca gaataaaaat agataatggc acattagaaa actaagtatc
      121 ccacagatga ttggataatg agaaaaatgt acctacaatc atctcatcag aaacaagcta
      181 catcatggaa tgttagttat ccaagcctgc acaataatga cattttaaac tgcaatcatc
      241 tattggccag catcacactg aaggcatcgt taaacattca agcaaagatt gctggcataa
      301 actattaaaa gacacacaca cacgcgcaca cacacacaga tacacacaca ctctctctca
      361 ttcatgcaca cttcccacat ttattaattt acctttgttc ccaaagataa gataccgntt
      421 tttttttccn cggggaacnt tatccaaatt tggccactna aagatgacag gtgnctttcc
      481 ccaggaatgg
//
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