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GenBank: N50641.1
FASTA Graphics
LOCUS N50641 490 bp mRNA linear EST 14-FEB-1996 DEFINITION yz18g09.s1 Soares_multiple_sclerosis_2NbHMSP Homo sapiens cDNA clone IMAGE:283456 3', mRNA sequence. ACCESSION N50641 VERSION N50641.1 DBLINK BioSample: SAMN00155002 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 490) AUTHORS Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M., Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M., Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F., Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and Wilson,R. TITLE The WashU-Merck EST Project JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: m13 -40 forward High quality sequence stop: 257. FEATURES Location/Qualifiers source 1..490 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="GDB:3901984" /db_xref="taxon:9606" /clone="IMAGE:283456" /sex="male" /tissue_type="multiple sclerosis lesions" /clone_lib="SAMN00155002 Soares_multiple_sclerosis_2NbHMSP" /dev_stage="Age 46" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker V_TYPE: phagemid; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA from 4 multiple sclerosis lesions from one patient was kindly provided by Dr. Kevin G. Becker (NINDS/NIH)." ORIGIN 1 nttttttttt acttttaaaa ttttatgttt aacaatttat tctattttaa aatatgtact 61 gtattttgaa cataactgca gaataaaaat agataatggc acattagaaa actaagtatc 121 ccacagatga ttggataatg agaaaaatgt acctacaatc atctcatcag aaacaagcta 181 catcatggaa tgttagttat ccaagcctgc acaataatga cattttaaac tgcaatcatc 241 tattggccag catcacactg aaggcatcgt taaacattca agcaaagatt gctggcataa 301 actattaaaa gacacacaca cacgcgcaca cacacacaga tacacacaca ctctctctca 361 ttcatgcaca cttcccacat ttattaattt acctttgttc ccaaagataa gataccgntt 421 tttttttccn cggggaacnt tatccaaatt tggccactna aagatgacag gtgnctttcc 481 ccaggaatgg //
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