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yv69c10.s1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE:247986 3', mRNA sequence

GenBank: N53758.1

FASTA Graphics 

LOCUS       N53758                   453 bp    mRNA    linear   EST 28-JAN-2011
DEFINITION  yv69c10.s1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone
            IMAGE:247986 3', mRNA sequence.
ACCESSION   N53758
VERSION     N53758.1
DBLINK      BioSample: SAMN00154876
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
            Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
            Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
            Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
            Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
            Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
            and Marra,M.
  TITLE     Generation and analysis of 280,000 human expressed sequence tags
  JOURNAL   Genome Res. 6 (9), 807-828 (1996)
   PUBMED   8889549
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 806   Std Error: 0.00
            Seq primer: m13 -40 forward
            High quality sequence stop: 403.
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:3797232"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:247986"
                     /sex="male"
                     /clone_lib="SAMN00154876 Soares fetal liver spleen 1NFLS"
                     /dev_stage="20 week-post conception fetus"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
                     with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
                     1st strand cDNA was primed with a Pac I - oligo(dT) primer
                     [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Pac I and cloned into the Pac I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization. Library
                     constructed by Bento Soares and M.Fatima Bonaldo."
ORIGIN      
        1 cacctaacct tccttggtac aaaaattaaa tctttcttaa tatagaatag gaagaatctg
       61 ctaattcaat aatactaatt tcttttttgc tgattaaaaa aggaatgaaa aaaatctcct
      121 catctagtca cttcctataa aatgcttgaa atgctttgtt caccattggc tttaataatt
      181 ccttccttca gttggtggaa aatgtatctg aatcagtaag aatctacttc tgccaattcc
      241 tttcaggaag acaaccttcc acctactgaa atcacacctc cactccaggc tgtagcattc
      301 ccagcctccc tccttcacct cagtgggacc ttgttatcct gatccgcaca gcattatgct
      361 gaactcccaa gataagggga acagaaaata accagtaaca ggtatacatg gtttaaacta
      421 tcattaaaat agttctataa aatagaaagc cag
//
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