LOCUS N53758 453 bp mRNA linear EST 28-JAN-2011
DEFINITION yv69c10.s1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone
IMAGE:247986 3', mRNA sequence.
ACCESSION N53758
VERSION N53758.1
DBLINK BioSample: SAMN00154876
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 453)
AUTHORS Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
and Marra,M.
TITLE Generation and analysis of 280,000 human expressed sequence tags
JOURNAL Genome Res. 6 (9), 807-828 (1996)
PUBMED 8889549
COMMENT Contact: Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: est@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Insert Length: 806 Std Error: 0.00
Seq primer: m13 -40 forward
High quality sequence stop: 403.
FEATURES Location/Qualifiers
source 1..453
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="GDB:3797232"
/db_xref="taxon:9606"
/clone="IMAGE:247986"
/sex="male"
/clone_lib="SAMN00154876 Soares fetal liver spleen 1NFLS"
/dev_stage="20 week-post conception fetus"
/lab_host="DH10B (ampicillin resistant)"
/note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
1st strand cDNA was primed with a Pac I - oligo(dT) primer
[5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Pac I and cloned into the Pac I
and Eco RI sites of the modified pT7T3 vector. Library
went through one round of normalization. Library
constructed by Bento Soares and M.Fatima Bonaldo."
ORIGIN
1 cacctaacct tccttggtac aaaaattaaa tctttcttaa tatagaatag gaagaatctg
61 ctaattcaat aatactaatt tcttttttgc tgattaaaaa aggaatgaaa aaaatctcct
121 catctagtca cttcctataa aatgcttgaa atgctttgtt caccattggc tttaataatt
181 ccttccttca gttggtggaa aatgtatctg aatcagtaag aatctacttc tgccaattcc
241 tttcaggaag acaaccttcc acctactgaa atcacacctc cactccaggc tgtagcattc
301 ccagcctccc tccttcacct cagtgggacc ttgttatcct gatccgcaca gcattatgct
361 gaactcccaa gataagggga acagaaaata accagtaaca ggtatacatg gtttaaacta
421 tcattaaaat agttctataa aatagaaagc cag
//