LOCUS N54092 221 bp mRNA linear EST 28-JAN-2011
DEFINITION yz02a07.r1 Soares_multiple_sclerosis_2NbHMSP Homo sapiens cDNA
clone IMAGE:281844 5', mRNA sequence.
ACCESSION N54092
VERSION N54092.1
DBLINK BioSample: SAMN00155002
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 221)
AUTHORS Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
and Marra,M.
TITLE Generation and analysis of 280,000 human expressed sequence tags
JOURNAL Genome Res. 6 (9), 807-828 (1996)
PUBMED 8889549
COMMENT Contact: Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: est@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Insert Length: 1228 Std Error: 0.00
Seq primer: T7
High quality sequence stop: 174.
FEATURES Location/Qualifiers
source 1..221
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="GDB:3900220"
/db_xref="taxon:9606"
/clone="IMAGE:281844"
/sex="male"
/tissue_type="multiple sclerosis lesions"
/clone_lib="SAMN00155002
Soares_multiple_sclerosis_2NbHMSP"
/dev_stage="Age 46"
/lab_host="DH10B (ampicillin resistant)"
/note="Vector: pT7T3D (Pharmacia) with a modified
polylinker V_TYPE: phagemid; Site_1: Not I; Site_2: Eco
RI; 1st strand cDNA was primed with a Not I - oligo(dT)
primer [5'
TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was size selected, ligated to Eco RI
adapters (Pharmacia), digested with Not I and cloned into
the Not I and Eco RI sites of a modified pT7T3 vector
(Pharmacia). Library went through one round of
normalization to a Cot = 5. Library constructed by Bento
Soares and M.Fatima Bonaldo. RNA from 4 multiple sclerosis
lesions from one patient was kindly provided by Dr. Kevin
G. Becker (NINDS/NIH)."
ORIGIN
1 gtagcagtgt cagcgtattt tttcttctca tcctggagcg tattcaagat cttcccaaca
61 caagaaaatt aataaaaaat ttatatatag gcagcagcaa aagagccatg ttcaaaatag
121 tcattatggg ctcaaataga aagaagactt ttaagtttta atccagttta tctgttgagt
181 tcngtgagct actgncctcc tgangctggg cactgtgtna g
//