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yz02a07.r1 Soares_multiple_sclerosis_2NbHMSP Homo sapiens cDNA clone IMAGE:281844 5', mRNA sequence

GenBank: N54092.1

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LOCUS       N54092                   221 bp    mRNA    linear   EST 28-JAN-2011
DEFINITION  yz02a07.r1 Soares_multiple_sclerosis_2NbHMSP Homo sapiens cDNA
            clone IMAGE:281844 5', mRNA sequence.
ACCESSION   N54092
VERSION     N54092.1
DBLINK      BioSample: SAMN00155002
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 221)
  AUTHORS   Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
            Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
            Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
            Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
            Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
            Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
            and Marra,M.
  TITLE     Generation and analysis of 280,000 human expressed sequence tags
  JOURNAL   Genome Res. 6 (9), 807-828 (1996)
   PUBMED   8889549
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 1228   Std Error: 0.00
            Seq primer: T7
            High quality sequence stop: 174.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:3900220"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:281844"
                     /sex="male"
                     /tissue_type="multiple sclerosis lesions"
                     /clone_lib="SAMN00155002
                     Soares_multiple_sclerosis_2NbHMSP"
                     /dev_stage="Age 46"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Vector: pT7T3D (Pharmacia) with a modified
                     polylinker V_TYPE: phagemid; Site_1: Not I; Site_2: Eco
                     RI; 1st strand cDNA was primed with a Not I - oligo(dT)
                     primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library went through one round of
                     normalization to a Cot = 5. Library constructed by Bento
                     Soares and M.Fatima Bonaldo. RNA from 4 multiple sclerosis
                     lesions from one patient was kindly provided by Dr. Kevin
                     G. Becker (NINDS/NIH)."
ORIGIN      
        1 gtagcagtgt cagcgtattt tttcttctca tcctggagcg tattcaagat cttcccaaca
       61 caagaaaatt aataaaaaat ttatatatag gcagcagcaa aagagccatg ttcaaaatag
      121 tcattatggg ctcaaataga aagaagactt ttaagtttta atccagttta tctgttgagt
      181 tcngtgagct actgncctcc tgangctggg cactgtgtna g
//
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