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yw91d12.r1 Soares_placenta_8to9weeks_2NbHP8to9W Homo sapiens cDNA clone IMAGE:259607 5', mRNA sequence

GenBank: N57193.1

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LOCUS       N57193                   221 bp    mRNA    linear   EST 22-FEB-1996
DEFINITION  yw91d12.r1 Soares_placenta_8to9weeks_2NbHP8to9W Homo sapiens cDNA
            clone IMAGE:259607 5', mRNA sequence.
ACCESSION   N57193
VERSION     N57193.1
DBLINK      BioSample: SAMN00154980
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 221)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: T7
            High quality sequence stop: 169.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:3889313"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:259607"
                     /clone_lib="SAMN00154980
                     Soares_placenta_8to9weeks_2NbHP8to9W"
                     /dev_stage="two placentae: one from 8 weeks and another
                     from 9 weeks post conception"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: placenta; Vector: pT7T3D (Pharmacia) with a
                     modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
                     strand cDNA was primed with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCGATTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library constructed by Bento Soares and
                     M.Fatima Bonaldo."
ORIGIN      
        1 gttattgaaa acaaatgttt ctcaacgagc tgcttcggaa caatataacg tattatgaag
       61 ctccgtacat tttacatgtt cttgcaatct tgacaggaca aaataagtcc tcaacttttt
      121 cctttgaatt ttgaaactga aaccattttc ttccttttcc agactctcct gcatcaagac
      181 cgggctgttt ggaagaaacc gtgtaaaaag tgcatgagca g
//
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