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za78c04.s1 Soares_fetal_lung_NbHL19W Homo sapiens cDNA clone IMAGE:298662 3', mRNA sequence

GenBank: N74313.1

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LOCUS       N74313                   140 bp    mRNA    linear   EST 19-MAR-1996
DEFINITION  za78c04.s1 Soares_fetal_lung_NbHL19W Homo sapiens cDNA clone
            IMAGE:298662 3', mRNA sequence.
ACCESSION   N74313
VERSION     N74313.1
DBLINK      BioSample: SAMN00155027
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 140)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: m13 -40 forward
            High quality sequence stop: 121.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:1243586"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:298662"
                     /clone_lib="SAMN00155027 Soares_fetal_lung_NbHL19W"
                     /dev_stage="19 weeks"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: lung; Vector: pT7T3D (Pharmacia) with a
                     modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
                     strand cDNA was primed with a Not I - oligo(dT) primer
                     [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAATTTTTTTTTTTTTTTTTT-3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library went through one round of
                     normalization to a Cot = 5. Library constructed by Bento
                     Soares and M.Fatima Bonaldo. This library was constructed
                     from the same fetus as the fetal heart library, Soares
                     fetal heart NbHH19W."
ORIGIN      
        1 ctggttccat tttattaaaa aacaaaacca aaagaaattt tgcagtgaaa tttcctggaa
       61 ttcacaaccc tcatttaaaa atccttatag ttgcaatatt ttttttttag ccagtacgta
      121 agaacacgat tgcncttatt
//
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