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zb30e06.s1 Soares_parathyroid_tumor_NbHPA Homo sapiens cDNA clone IMAGE:305122 3', mRNA sequence

GenBank: N92611.1

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LOCUS       N92611                   398 bp    mRNA    linear   EST 05-APR-1996
DEFINITION  zb30e06.s1 Soares_parathyroid_tumor_NbHPA Homo sapiens cDNA clone
            IMAGE:305122 3', mRNA sequence.
ACCESSION   N92611
VERSION     N92611.1
DBLINK      BioSample: SAMN00155028
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: ETPrimer
            High quality sequence stop: 296.
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:1248532"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:305122"
                     /tissue_type="parathyroid tumor"
                     /clone_lib="SAMN00155028 Soares_parathyroid_tumor_NbHPA"
                     /dev_stage="adult"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: parathyroid gland; Vector: pT7T3D
                     (Pharmacia) with a modified polylinker; Site_1: Not I;
                     Site_2: Eco RI; 1st strand cDNA was primed with a Not I -
                     oligo(dT) primer
                     [5'-
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCACCAATTTTTTTTTTTTTTTTTTTTTTTT
                     T-3'], double-stranded cDNA was size selected, ligated to
                     Eco RI adapters (Pharmacia), digested with Not I and
                     cloned into the Not I and Eco RI sites of a modified pT7T3
                     vector (Pharmacia). Library went through one round of
                     normalization to a Cot = 5. Library constructed by Bento
                     Soares and M.Fatima Bonaldo. RNA from sporadic parathyroid
                     adenomas was kindly provided by Dr. Stephen Marx, National
                     Institute of Diabetes and Digestive and Kidney Diseases,
                     NIH."
ORIGIN      
        1 tttttttcac agtaggaaag gcactcgttt atttatttta acagcaaata ttatggtctt
       61 ttatccttaa gttccattac atcaaaacaa taaagtatta acatttaact taaggtaatt
      121 cagtgtcctc agcttttaaa gaaataaata ctttaaaaca atgaaaataa atcagtttgc
      181 tttcagaaac tacacactaa atgaatttct cttcttggtt gatgatggcc aaaatcccaa
      241 ctgtagaacc atcctcagag aacttgtcat gtaaaaacac ttggttcaat ttagatatca
      301 tcaatgnttc atcatcatcc caatacgtca antggatttc acacactcca ggccaaatgt
      361 gntgttcatt gattttgcng tctggantcg catatgct
//
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