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GenBank: N92611.1
FASTA Graphics
LOCUS N92611 398 bp mRNA linear EST 05-APR-1996 DEFINITION zb30e06.s1 Soares_parathyroid_tumor_NbHPA Homo sapiens cDNA clone IMAGE:305122 3', mRNA sequence. ACCESSION N92611 VERSION N92611.1 DBLINK BioSample: SAMN00155028 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 398) AUTHORS Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M., Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M., Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F., Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and Wilson,R. TITLE The WashU-Merck EST Project JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: ETPrimer High quality sequence stop: 296. FEATURES Location/Qualifiers source 1..398 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="GDB:1248532" /db_xref="taxon:9606" /clone="IMAGE:305122" /tissue_type="parathyroid tumor" /clone_lib="SAMN00155028 Soares_parathyroid_tumor_NbHPA" /dev_stage="adult" /lab_host="DH10B (ampicillin resistant)" /note="Organ: parathyroid gland; Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5'- TGTTACCAATCTGAAGTGGGAGCGGCCGCACCAATTTTTTTTTTTTTTTTTTTTTTTT T-3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA from sporadic parathyroid adenomas was kindly provided by Dr. Stephen Marx, National Institute of Diabetes and Digestive and Kidney Diseases, NIH." ORIGIN 1 tttttttcac agtaggaaag gcactcgttt atttatttta acagcaaata ttatggtctt 61 ttatccttaa gttccattac atcaaaacaa taaagtatta acatttaact taaggtaatt 121 cagtgtcctc agcttttaaa gaaataaata ctttaaaaca atgaaaataa atcagtttgc 181 tttcagaaac tacacactaa atgaatttct cttcttggtt gatgatggcc aaaatcccaa 241 ctgtagaacc atcctcagag aacttgtcat gtaaaaacac ttggttcaat ttagatatca 301 tcaatgnttc atcatcatcc caatacgtca antggatttc acacactcca ggccaaatgt 361 gntgttcatt gattttgcng tctggantcg catatgct //
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