LOCUS N95316 376 bp mRNA linear EST 28-JAN-2011
DEFINITION zb61d11.s1 Soares_fetal_lung_NbHL19W Homo sapiens cDNA clone
IMAGE:308085 3', mRNA sequence.
ACCESSION N95316
VERSION N95316.1
DBLINK BioSample: SAMN00155027
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 376)
AUTHORS Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
and Marra,M.
TITLE Generation and analysis of 280,000 human expressed sequence tags
JOURNAL Genome Res. 6 (9), 807-828 (1996)
PUBMED 8889549
COMMENT Contact: Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: est@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Insert Length: 444 Std Error: 0.00
Seq primer: ETPrimer
High quality sequence stop: 261.
FEATURES Location/Qualifiers
source 1..376
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="GDB:1251498"
/db_xref="taxon:9606"
/clone="IMAGE:308085"
/clone_lib="SAMN00155027 Soares_fetal_lung_NbHL19W"
/dev_stage="19 weeks"
/lab_host="DH10B (ampicillin resistant)"
/note="Organ: lung; Vector: pT7T3D (Pharmacia) with a
modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
strand cDNA was primed with a Not I - oligo(dT) primer
[5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAATTTTTTTTTTTTTTTTTT-3'],
double-stranded cDNA was size selected, ligated to Eco RI
adapters (Pharmacia), digested with Not I and cloned into
the Not I and Eco RI sites of a modified pT7T3 vector
(Pharmacia). Library went through one round of
normalization to a Cot = 5. Library constructed by Bento
Soares and M.Fatima Bonaldo. This library was constructed
from the same fetus as the fetal heart library, Soares
fetal heart NbHH19W."
ORIGIN
1 tttttttttt ttttttttta agaattcaag ttagttttat tcaaaggtct tactgagaat
61 cctagaccca ggtcccagcc cgggagcagc cttagagagc tcctgtttga ctgcccggct
121 cagtgtctcg gcccactgct tggttacggg ggccggggct caggacctgc acagtcatgg
181 ctccgaagtt acattacagc agaatcacat caaggtttgg gcataagagc acttctggtt
241 acagattaca gaagcataat cgctaacttg gccaacattt tctcatgtgc acgaaaagcc
301 aaggaccagg gtcttcactt ttaaggaata cagtgatcag gcaacagacg ggggcgtgtg
361 tntttcctgt tttgtt
//