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zb61d11.s1 Soares_fetal_lung_NbHL19W Homo sapiens cDNA clone IMAGE:308085 3', mRNA sequence

GenBank: N95316.1

FASTA Graphics 

LOCUS       N95316                   376 bp    mRNA    linear   EST 28-JAN-2011
DEFINITION  zb61d11.s1 Soares_fetal_lung_NbHL19W Homo sapiens cDNA clone
            IMAGE:308085 3', mRNA sequence.
ACCESSION   N95316
VERSION     N95316.1
DBLINK      BioSample: SAMN00155027
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
            Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
            Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
            Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
            Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
            Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
            and Marra,M.
  TITLE     Generation and analysis of 280,000 human expressed sequence tags
  JOURNAL   Genome Res. 6 (9), 807-828 (1996)
   PUBMED   8889549
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 444   Std Error: 0.00
            Seq primer: ETPrimer
            High quality sequence stop: 261.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:1251498"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:308085"
                     /clone_lib="SAMN00155027 Soares_fetal_lung_NbHL19W"
                     /dev_stage="19 weeks"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: lung; Vector: pT7T3D (Pharmacia) with a
                     modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
                     strand cDNA was primed with a Not I - oligo(dT) primer
                     [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAATTTTTTTTTTTTTTTTTT-3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library went through one round of
                     normalization to a Cot = 5. Library constructed by Bento
                     Soares and M.Fatima Bonaldo. This library was constructed
                     from the same fetus as the fetal heart library, Soares
                     fetal heart NbHH19W."
ORIGIN      
        1 tttttttttt ttttttttta agaattcaag ttagttttat tcaaaggtct tactgagaat
       61 cctagaccca ggtcccagcc cgggagcagc cttagagagc tcctgtttga ctgcccggct
      121 cagtgtctcg gcccactgct tggttacggg ggccggggct caggacctgc acagtcatgg
      181 ctccgaagtt acattacagc agaatcacat caaggtttgg gcataagagc acttctggtt
      241 acagattaca gaagcataat cgctaacttg gccaacattt tctcatgtgc acgaaaagcc
      301 aaggaccagg gtcttcactt ttaaggaata cagtgatcag gcaacagacg ggggcgtgtg
      361 tntttcctgt tttgtt
//
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