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ya50a03.r3 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE:66316 5', mRNA sequence

GenBank: T66795.1

FASTA Graphics 

LOCUS       T66795                   442 bp    mRNA    linear   EST 07-MAR-1995
DEFINITION  ya50a03.r3 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone
            IMAGE:66316 5', mRNA sequence.
ACCESSION   T66795
VERSION     T66795.1
DBLINK      BioSample: SAMN00154876
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Other_ESTs: ya50a03.s2.exp
            Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            Source: IMAGE Consortium, LLNL
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: T7.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:66316"
                     /sex="male"
                     /clone_lib="SAMN00154876 Soares fetal liver spleen 1NFLS"
                     /dev_stage="20 week-post conception fetus"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
                     with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
                     1st strand cDNA was primed with a Pac I - oligo(dT) primer
                     [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Pac I and cloned into the Pac I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization. Library
                     constructed by Bento Soares and M.Fatima Bonaldo."
ORIGIN      
        1 ctttctctac atggttgttc atgtagaact ttgggcaact cactgtgccc tcaagttatg
       61 agtccaataa cctgttgctc cagatgcttg atttcctcaa ctccagtgcc cttcaggtga
      121 ggtccacctc actttgctga tagtggcaga ggcagtcaaa tgcctaggcc gatggggcgg
      181 gtcccaatga aacccccctt caaaccaaag acagttcaaa gcctgaaatc caagctacaa
      241 gtcttggata aatccatgga ccaaattgag aacctctctt ttcgtttggc atgctttcct
      301 ctgattaatc cccaccttcc acctatttta catataccta ctcttcccta agtggtttct
      361 tgcactgtgt ttcttaccta cctttgagtg ggtgcctttg ttttagcctt tcttgcatac
      421 tcataaacca atcagcatgc ac
//
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