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GenBank: T66795.1
FASTA Graphics
LOCUS T66795 442 bp mRNA linear EST 07-MAR-1995 DEFINITION ya50a03.r3 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE:66316 5', mRNA sequence. ACCESSION T66795 VERSION T66795.1 DBLINK BioSample: SAMN00154876 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 442) AUTHORS Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M., Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M., Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F., Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and Wilson,R. TITLE The WashU-Merck EST Project JOURNAL Unpublished COMMENT Other_ESTs: ya50a03.s2.exp Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu Source: IMAGE Consortium, LLNL This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: T7. FEATURES Location/Qualifiers source 1..442 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:66316" /sex="male" /clone_lib="SAMN00154876 Soares fetal liver spleen 1NFLS" /dev_stage="20 week-post conception fetus" /lab_host="DH10B (ampicillin resistant)" /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Pac I; Site_2: Eco RI; 1st strand cDNA was primed with a Pac I - oligo(dT) primer [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Pac I and cloned into the Pac I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by Bento Soares and M.Fatima Bonaldo." ORIGIN 1 ctttctctac atggttgttc atgtagaact ttgggcaact cactgtgccc tcaagttatg 61 agtccaataa cctgttgctc cagatgcttg atttcctcaa ctccagtgcc cttcaggtga 121 ggtccacctc actttgctga tagtggcaga ggcagtcaaa tgcctaggcc gatggggcgg 181 gtcccaatga aacccccctt caaaccaaag acagttcaaa gcctgaaatc caagctacaa 241 gtcttggata aatccatgga ccaaattgag aacctctctt ttcgtttggc atgctttcct 301 ctgattaatc cccaccttcc acctatttta catataccta ctcttcccta agtggtttct 361 tgcactgtgt ttcttaccta cctttgagtg ggtgcctttg ttttagcctt tcttgcatac 421 tcataaacca atcagcatgc ac //
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