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yd31f05.r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE:109857 5', mRNA sequence

GenBank: T84247.1

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LOCUS       T84247                   460 bp    mRNA    linear   EST 16-MAR-1995
DEFINITION  yd31f05.r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone
            IMAGE:109857 5', mRNA sequence.
ACCESSION   T84247
VERSION     T84247.1
DBLINK      BioSample: SAMN00154876
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            Insert Size: 673
            High quality sequence stops: 328 Source: IMAGE Consortium, LLNL
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 673   Std Error: 0.00
            Seq primer: M13RP1
            High quality sequence stop: 328.
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:465474"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:109857"
                     /sex="male"
                     /clone_lib="SAMN00154876 Soares fetal liver spleen 1NFLS"
                     /dev_stage="20 week-post conception fetus"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
                     with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
                     1st strand cDNA was primed with a Pac I - oligo(dT) primer
                     [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Pac I and cloned into the Pac I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization. Library
                     constructed by Bento Soares and M.Fatima Bonaldo."
ORIGIN      
        1 atattgtcag tttttcatcg tcgtccctag cctgccaaca gccatctgcc cagacagccg
       61 cagtgaggat gagcgtcctg gcagagacgc agttgtctct gggcgcttgc agagccacga
      121 accccagacc tgtttgtatc atccgggctc cttccgggca gaaacaactg aaaatgcact
      181 tcagacccac ttatttctgc cacatctgag tcggcctgag atagactttt ccctctaaac
      241 tgggagaata tcacagtggt ttttgttagc agaaaatgca ctccagcctc tgtactcatc
      301 taaggctgct tatttttgat atttgtgttc agtctgttaa atgggattac tttcactttt
      361 taattaactg tttggcttta ggtaattggg ctttgttgga ggaaggtggg gaaaaaaatg
      421 ggtttttggn tctttcaact tccctttgnc atgccngggc
//
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