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za56b11.r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE:296541 5', mRNA sequence

GenBank: W01079.1

FASTA Graphics 

LOCUS       W01079                   446 bp    mRNA    linear   EST 18-APR-1996
DEFINITION  za56b11.r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone
            IMAGE:296541 5', mRNA sequence.
ACCESSION   W01079
VERSION     W01079.1
DBLINK      BioSample: SAMN00154876
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: mob.REGA+ET.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:1241462"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:296541"
                     /sex="male"
                     /clone_lib="SAMN00154876 Soares fetal liver spleen 1NFLS"
                     /dev_stage="20 week-post conception fetus"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
                     with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
                     1st strand cDNA was primed with a Pac I - oligo(dT) primer
                     [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Pac I and cloned into the Pac I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization. Library
                     constructed by Bento Soares and M.Fatima Bonaldo."
ORIGIN      
        1 attttaaatc tgagagctcg ctttgagtga ctcgggtttt gtgattgcct ctgaagccta
       61 tgtatgccat ggaggcacta acaaactctg aggtttccga aatcagaagc gaaaaaatca
      121 gtgaataaac catcatcttg ccactacccc ctcctgaagc cacagcaggg tttcaggttc
      181 caatcagaac tgttggcaag gtgacatttc catgcataaa tgcgatccac agaaggtcct
      241 ggtggtattt gtaacttttt gcaaggcatt tttttatata tatttttgtg cacatttttt
      301 tttacgtttc tttagaaaac aaatgtattt caaaatatat ttatagtcga acaattcata
      361 tatttgaagt ggagccatat gaatgtcagt agtttatact tctctattat ctcaaactac
      421 tggcaatttg taaagaaata tatatg
//
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