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GenBank: W02546.1
FASTA Graphics
LOCUS W02546 429 bp mRNA linear EST 18-APR-1996 DEFINITION za49d07.r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE:295885 5', mRNA sequence. ACCESSION W02546 VERSION W02546.1 DBLINK BioSample: SAMN00154876 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 429) AUTHORS Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M., Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M., Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F., Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and Wilson,R. TITLE The WashU-Merck EST Project JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: mob.REGA+ET High quality sequence stop: 290. FEATURES Location/Qualifiers source 1..429 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="GDB:1240806" /db_xref="taxon:9606" /clone="IMAGE:295885" /sex="male" /clone_lib="SAMN00154876 Soares fetal liver spleen 1NFLS" /dev_stage="20 week-post conception fetus" /lab_host="DH10B (ampicillin resistant)" /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Pac I; Site_2: Eco RI; 1st strand cDNA was primed with a Pac I - oligo(dT) primer [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Pac I and cloned into the Pac I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by Bento Soares and M.Fatima Bonaldo." ORIGIN 1 nttctcgtct tgaagacaca ctgcagtaag tttactttta tcaatattta aaagaaaatt 61 accataagta tgctagggac tgttaattct gatgcattta aacttttagt tttgcttaga 121 aaacttactt atacagattt tttttaaaga aattcaagct tttcatatgt attgtattat 181 cattcctccg tgttttagct gcaacaaata tgaagtggat tgaagctaaa atgaaagagc 241 tgggagtgag cacaaatgca aattataaga ttactttcat gttggatagt gctgctatga 301 taacagtaca tactccaagg agaggattaa tagacgtaag angtccatgg ttaattccta 361 tttaatgncc accatgggtt aatttcccag gggacctttt aaggttaaac cnaagggggt 421 aataggggt //
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