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GenBank: W02790.1
FASTA Graphics
LOCUS W02790 399 bp mRNA linear EST 18-APR-1996 DEFINITION zc64b11.s1 Soares_fetal_heart_NbHH19W Homo sapiens cDNA clone IMAGE:327069 3', mRNA sequence. ACCESSION W02790 VERSION W02790.1 DBLINK BioSample: SAMN00155035 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 399) AUTHORS Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M., Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M., Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F., Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and Wilson,R. TITLE The WashU-Merck EST Project JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: mob.REGA+ET High quality sequence stop: 369. FEATURES Location/Qualifiers source 1..399 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="GDB:1261271" /db_xref="taxon:9606" /clone="IMAGE:327069" /sex="unknown" /clone_lib="SAMN00155035 Soares_fetal_heart_NbHH19W" /dev_stage="19 weeks" /lab_host="DH10B (ampicillin resistant)" /note="Organ: heart; Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATCTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by M.Fatima Bonaldo. This library was constructed from the same fetus as the fetal lung library, Soares fetal lung NbHL19W." ORIGIN 1 aaagagtcat aaaagtttat tatatgaaag aaataacatg gcttctgtac atctatttac 61 agaacagaga cttatttaca gtgagatgga ggccttggtg ttggtgaggg acagacagag 121 agtgaggagg cgtgacagcc tgcctcccat ggctcgaaaa cagggaaccc cagcaggaag 181 gggagcagca aaagctggtg ggggaaccgg cgttcctgtt cttcctcaat ggagacctgg 241 tgagcatgta gcaggtggga tggaacccag agtccacgca ggaccctgga ggtgcaaggg 301 agatgcttga gttgaaccca gaacattctc gggttgatca cagtgtcctt ggggtgctct 361 ggggaaagtt gtgtgcccct ctgcccctga cgctgtccg //
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