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zd58a05.s1 Soares_fetal_heart_NbHH19W Homo sapiens cDNA clone IMAGE:344816 3', mRNA sequence

GenBank: W70203.1

FASTA Graphics 

LOCUS       W70203                   346 bp    mRNA    linear   EST 16-OCT-1996
DEFINITION  zd58a05.s1 Soares_fetal_heart_NbHH19W Homo sapiens cDNA clone
            IMAGE:344816 3', mRNA sequence.
ACCESSION   W70203
VERSION     W70203.1
DBLINK      BioSample: SAMN00155035
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 644   Std Error: 0.00
            Seq primer: mob.REGA+ET.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:1270191"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:344816"
                     /sex="unknown"
                     /clone_lib="SAMN00155035 Soares_fetal_heart_NbHH19W"
                     /dev_stage="19 weeks"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: heart; Vector: pT7T3D (Pharmacia) with a
                     modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
                     strand cDNA was primed with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCATCTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library went through one round of
                     normalization to a Cot = 5. Library constructed by
                     M.Fatima Bonaldo. This library was constructed from the
                     same fetus as the fetal lung library, Soares fetal lung
                     NbHL19W."
ORIGIN      
        1 aaggtatgta tgaccttgtt tattatctat catagacatc aagatgatca tagttaatac
       61 caatttaagc tttacagaat actgttttag gcccaatatt gatatattaa atgaaggtat
      121 cagagaatct tgtatttatg gcatcaggtt ataaagatct attcaaaacc atttttgtca
      181 aagtttaaac actggaacaa aagtcaaatt gtttctaaat gagacacaaa atgattcttg
      241 ctaatagtac aaattttgtc ccatgggtaa tactattgtc tttttctttt ttaaaaaaat
      301 tatttcgatt tttattttag attcaggaaa cacatngggc aggttt
//
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