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zh51a07.s1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE:415572 3', mRNA sequence

GenBank: W80706.1

FASTA Graphics 

LOCUS       W80706                   186 bp    mRNA    linear   EST 28-JAN-2011
DEFINITION  zh51a07.s1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA
            clone IMAGE:415572 3', mRNA sequence.
ACCESSION   W80706
VERSION     W80706.1
DBLINK      BioSample: SAMN00155062
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 186)
  AUTHORS   Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
            Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
            Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
            Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
            Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
            Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
            and Marra,M.
  TITLE     Generation and analysis of 280,000 human expressed sequence tags
  JOURNAL   Genome Res. 6 (9), 807-828 (1996)
   PUBMED   8889549
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 917   Std Error: 0.00
            Seq primer: mob.REGA+ET.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:1324042"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:415572"
                     /sex="male"
                     /clone_lib="SAMN00155062
                     Soares_fetal_liver_spleen_1NFLS_S1"
                     /dev_stage="20 week-post conception fetus"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
                     with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
                     This is a subtracted version of the original Soares fetal
                     liver spleen 1NFLS library. 1st strand cDNA was primed
                     with a Pac I - oligo(dT) primer [5'
                     AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Pac I and cloned into the Pac I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization. Library
                     constructed by Bento Soares and M.Fatima Bonaldo."
ORIGIN      
        1 gcttgttttt gtttttggtg aagaaaacta atctgggcaa atagctcatg atgaaatttg
       61 accagaaaat ttctgaaagt gtccagtagt gtttttgtct ctctgatccc actgagccac
      121 tccttcacaa tcttgggggc tgcatgccac ctccaaggta tgagagcatc acctggcact
      181 tctgga
//
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