LOCUS W80706 186 bp mRNA linear EST 28-JAN-2011
DEFINITION zh51a07.s1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA
clone IMAGE:415572 3', mRNA sequence.
ACCESSION W80706
VERSION W80706.1
DBLINK BioSample: SAMN00155062
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 186)
AUTHORS Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
and Marra,M.
TITLE Generation and analysis of 280,000 human expressed sequence tags
JOURNAL Genome Res. 6 (9), 807-828 (1996)
PUBMED 8889549
COMMENT Contact: Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: est@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Insert Length: 917 Std Error: 0.00
Seq primer: mob.REGA+ET.
FEATURES Location/Qualifiers
source 1..186
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="GDB:1324042"
/db_xref="taxon:9606"
/clone="IMAGE:415572"
/sex="male"
/clone_lib="SAMN00155062
Soares_fetal_liver_spleen_1NFLS_S1"
/dev_stage="20 week-post conception fetus"
/lab_host="DH10B (ampicillin resistant)"
/note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
This is a subtracted version of the original Soares fetal
liver spleen 1NFLS library. 1st strand cDNA was primed
with a Pac I - oligo(dT) primer [5'
AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Pac I and cloned into the Pac I
and Eco RI sites of the modified pT7T3 vector. Library
went through one round of normalization. Library
constructed by Bento Soares and M.Fatima Bonaldo."
ORIGIN
1 gcttgttttt gtttttggtg aagaaaacta atctgggcaa atagctcatg atgaaatttg
61 accagaaaat ttctgaaagt gtccagtagt gtttttgtct ctctgatccc actgagccac
121 tccttcacaa tcttgggggc tgcatgccac ctccaaggta tgagagcatc acctggcact
181 tctgga
//