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ze93d07.s1 Soares_fetal_heart_NbHH19W Homo sapiens cDNA clone IMAGE:366541 3', mRNA sequence

GenBank: AA026626.1

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LOCUS       AA026626                 447 bp    mRNA    linear   EST 28-JAN-2011
DEFINITION  ze93d07.s1 Soares_fetal_heart_NbHH19W Homo sapiens cDNA clone
            IMAGE:366541 3', mRNA sequence.
ACCESSION   AA026626
VERSION     AA026626.1
DBLINK      BioSample: SAMN00155035
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
            Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
            Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
            Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
            Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
            Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
            and Marra,M.
  TITLE     Generation and analysis of 280,000 human expressed sequence tags
  JOURNAL   Genome Res. 6 (9), 807-828 (1996)
   PUBMED   8889549
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 1113   Std Error: 0.00
            Seq primer: -40M13 fwd. from Amersham.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:1283245"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:366541"
                     /sex="unknown"
                     /clone_lib="SAMN00155035 Soares_fetal_heart_NbHH19W"
                     /dev_stage="19 weeks"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: heart; Vector: pT7T3D (Pharmacia) with a
                     modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
                     strand cDNA was primed with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCATCTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library went through one round of
                     normalization to a Cot = 5. Library constructed by
                     M.Fatima Bonaldo. This library was constructed from the
                     same fetus as the fetal lung library, Soares fetal lung
                     NbHL19W."
ORIGIN      
        1 ttgttggaat gaatggcagc cctccctgga gaggcagcct gtttactgaa tacagaggat
       61 acgtttacaa actgaatacg cataataaat aactgcacat tctccatcca caggccatgg
      121 catgaaggcc caagtgggtc tatcaaaggc ccacatctcc aaaccccctg tcctgccctc
      181 aggaccaggc ccaccctggn aaagagagaa cgtaagcccc aggnttcagg tccccagaga
      241 cacttgggga actgggggga aattctgagg ccatggggct tggttctcca ctgcctcctg
      301 cccagggnat ttnggggacg gtaggaggat gtgtctaagg catagtcgac ttggcacaga
      361 gtggtctctt tagttttggt ttcccactgg aggtggcaca tgcaggaaaa gggcctggcc
      421 aggctgccga ccggaaaagc tgagtgg
//
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