LOCUS AA026626 447 bp mRNA linear EST 28-JAN-2011
DEFINITION ze93d07.s1 Soares_fetal_heart_NbHH19W Homo sapiens cDNA clone
IMAGE:366541 3', mRNA sequence.
ACCESSION AA026626
VERSION AA026626.1
DBLINK BioSample: SAMN00155035
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 447)
AUTHORS Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
and Marra,M.
TITLE Generation and analysis of 280,000 human expressed sequence tags
JOURNAL Genome Res. 6 (9), 807-828 (1996)
PUBMED 8889549
COMMENT Contact: Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: est@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Insert Length: 1113 Std Error: 0.00
Seq primer: -40M13 fwd. from Amersham.
FEATURES Location/Qualifiers
source 1..447
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="GDB:1283245"
/db_xref="taxon:9606"
/clone="IMAGE:366541"
/sex="unknown"
/clone_lib="SAMN00155035 Soares_fetal_heart_NbHH19W"
/dev_stage="19 weeks"
/lab_host="DH10B (ampicillin resistant)"
/note="Organ: heart; Vector: pT7T3D (Pharmacia) with a
modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
strand cDNA was primed with a Not I - oligo(dT) primer [5'
TGTTACCAATCTGAAGTGGGAGCGGCCGCATCTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was size selected, ligated to Eco RI
adapters (Pharmacia), digested with Not I and cloned into
the Not I and Eco RI sites of a modified pT7T3 vector
(Pharmacia). Library went through one round of
normalization to a Cot = 5. Library constructed by
M.Fatima Bonaldo. This library was constructed from the
same fetus as the fetal lung library, Soares fetal lung
NbHL19W."
ORIGIN
1 ttgttggaat gaatggcagc cctccctgga gaggcagcct gtttactgaa tacagaggat
61 acgtttacaa actgaatacg cataataaat aactgcacat tctccatcca caggccatgg
121 catgaaggcc caagtgggtc tatcaaaggc ccacatctcc aaaccccctg tcctgccctc
181 aggaccaggc ccaccctggn aaagagagaa cgtaagcccc aggnttcagg tccccagaga
241 cacttgggga actgggggga aattctgagg ccatggggct tggttctcca ctgcctcctg
301 cccagggnat ttnggggacg gtaggaggat gtgtctaagg catagtcgac ttggcacaga
361 gtggtctctt tagttttggt ttcccactgg aggtggcaca tgcaggaaaa gggcctggcc
421 aggctgccga ccggaaaagc tgagtgg
//