LOCUS AA043610 230 bp mRNA linear EST 28-JAN-2011
DEFINITION zk49f04.r1 Soares_pregnant_uterus_NbHPU Homo sapiens cDNA clone
IMAGE:486175 5', mRNA sequence.
ACCESSION AA043610
VERSION AA043610.1
DBLINK BioSample: SAMN00155083
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 230)
AUTHORS Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
and Marra,M.
TITLE Generation and analysis of 280,000 human expressed sequence tags
JOURNAL Genome Res. 6 (9), 807-828 (1996)
PUBMED 8889549
COMMENT Contact: Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: est@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Insert Length: 1592 Std Error: 0.00
Seq primer: -28M13 rev2 from Amersham
High quality sequence stop: 158.
FEATURES Location/Qualifiers
source 1..230
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="GDB:3759889"
/db_xref="taxon:9606"
/clone="IMAGE:486175"
/sex="female"
/clone_lib="SAMN00155083 Soares_pregnant_uterus_NbHPU"
/dev_stage="adult"
/lab_host="DH10B"
/note="Organ: uterus; Vector: pT7T3D-PacI; Site_1: Not I;
Site_2: Eco RI; 1st strand cDNA was primed with a Not I -
oligo(dT) primer [5'
AACTGGAAGAATTCGCGGCCGCCTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Not I and cloned into the Not I
and Eco RI sites of the modified pT7T3 vector. Library
went through one round of normalization. Library
constructed by M. Fatima Bonaldo."
ORIGIN
1 ggtaaattga gcagttcatg acccaggata tctgaaaata ttctactggc ctgtaatcta
61 ccagtggtgc tcaatgcaaa tagtagacat ttgtgtggaa atcataccag ttgttcattg
121 atgggatttt tgtttgactc cttaccaata gcctgaattt gaggagggaa tgattggtag
181 ccaagggatg ggggaaagaa gtaggttcng ttttgttttg ttttaaactt
//