LOCUS AA142989 487 bp mRNA linear EST 28-JAN-2011
DEFINITION zl48c04.r1 Soares_pregnant_uterus_NbHPU Homo sapiens cDNA clone
IMAGE:505158 5', mRNA sequence.
ACCESSION AA142989
VERSION AA142989.1
DBLINK BioSample: SAMN00155083
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 487)
AUTHORS Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
and Marra,M.
TITLE Generation and analysis of 280,000 human expressed sequence tags
JOURNAL Genome Res. 6 (9), 807-828 (1996)
PUBMED 8889549
COMMENT Contact: Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: est@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Insert Length: 565 Std Error: 0.00
Seq primer: -28M13 rev2 from Amersham
High quality sequence stop: 422.
FEATURES Location/Qualifiers
source 1..487
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="GDB:3810146"
/db_xref="taxon:9606"
/clone="IMAGE:505158"
/sex="female"
/clone_lib="SAMN00155083 Soares_pregnant_uterus_NbHPU"
/dev_stage="adult"
/lab_host="DH10B"
/note="Organ: uterus; Vector: pT7T3D-PacI; Site_1: Not I;
Site_2: Eco RI; 1st strand cDNA was primed with a Not I -
oligo(dT) primer [5'
AACTGGAAGAATTCGCGGCCGCCTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Not I and cloned into the Not I
and Eco RI sites of the modified pT7T3 vector. Library
went through one round of normalization. Library
constructed by M. Fatima Bonaldo."
ORIGIN
1 ttgaaattaa agggtgtctg gatgccatgt taattttatt gagcatgaga aagtatttga
61 agaaactgtt gcacaacata tgatgaaatt ttgatttctc tgaatctcag agtaatttaa
121 atgggcaaat ccaatgagtt gatgtaaccc atctacattt tgtggctatt tcatgtataa
181 aatgaaaggc tttagttatt tctagaggag tcccatccaa cactatttgg cgactgttaa
241 tatgttataa ttattagaca gtggaaaagc ctttattgac taattgattt aacctcagtc
301 caaagaacag tctctatact tgccctcatt tactgatagg ctgctactag ttaccatttg
361 attgtcttga ctttcgtctt cagttgaatt ggtgaaaaca tcaacaaaaa caaaacccac
421 tgtgtcttaa aataaaatgt tctgcattgt agtcaataaa gggctaagat gtaaaccatg
481 cnaaaaa
//